Construct: ORF TRCN0000470663
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003696.1_s317c1
- Derived from:
- ccsbBroadEn_03241
- DNA Barcode:
- TAACACCCAATTTACCAAAGATTT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRBN (51185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470663
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51185 | CRBN | cereblon | NM_016302.3 | 98.8% | 98.8% | 1312_1326del |
| 2 | human | 51185 | CRBN | cereblon | NM_001173482.1 | 98.6% | 98.6% | 66_67insGCA;1309_1323del |
| 3 | human | 51185 | CRBN | cereblon | XM_011533791.3 | 88.9% | 88.6% | 1015_1016ins132;1180_1194del |
| 4 | human | 51185 | CRBN | cereblon | XM_005265202.4 | 84.6% | 84.6% | 0_1ins189;1123_1137del |
| 5 | human | 51185 | CRBN | cereblon | XM_024453551.1 | 64.6% | 63.6% | (many diffs) |
| 6 | human | 51185 | CRBN | cereblon | XM_011533793.2 | 60.7% | 59.2% | (many diffs) |
| 7 | human | 51185 | CRBN | cereblon | XM_011533794.2 | 60.7% | 59.2% | (many diffs) |
| 8 | human | 51185 | CRBN | cereblon | XR_940448.3 | 42.9% | 1_14del;849_850ins116;1210_2667del | |
| 9 | mouse | 58799 | Crbn | cereblon | NM_175357.3 | 88.5% | 94.1% | (many diffs) |
| 10 | mouse | 58799 | Crbn | cereblon | NM_021449.3 | 88.3% | 93.9% | (many diffs) |
| 11 | mouse | 58799 | Crbn | cereblon | XR_001785159.1 | 44.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1377
- ORF length:
- 1311
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggcgaagga gatcagcagg acgctgcgca caacatgggc aaccacctgc 121 cgctcctgcc tgcagagagt gaggaagaag atgaaatgga agttgaagac caggatagta 181 aagaagccaa aaaaccaaac atcataaatt ttgacaccag tctgccgaca tcacatacat 241 acctaggtgc tgatatggaa gaatttcatg gcaggacttt gcacgatgac gacagctgtc 301 aggtgattcc agttcttcca caagtgatga tgatcctgat tcccggacag acattacctc 361 ttcagctttt tcaccctcaa gaagtcagta tggtgcggaa tttaattcag aaagatagaa 421 cctttgctgt tcttgcatac agcaatgtac aggaaaggga agcacagttt ggaacaacag 481 cagagatata tgcctatcga gaagaacagg attttggaat tgagatagtg aaagtgaaag 541 caattggaag acaaaggttc aaagtccttg agctaagaac acagtcagat ggaatccagc 601 aagctaaagt gcaaattctt cccgaatgtg tgttgccttc aaccatgtct gcagttcaat 661 tagaatccct caataagtgc cagatatttc cttcaaaacc tgtctcaaga gaagaccaat 721 gttcatataa atggtggcag aaataccaga agagaaagtt tcattgtgca aatctaactt 781 catggcctcg ctggctgtat tccttatatg atgctgagac cttaatggac agaatcaaga 841 aacagctacg tgaatgggat gaaaatctaa aagatgattc tcttccttca aatccaatag 901 atttttctta cagagtagct gcttgTCTTC CTATTGATGA TGTATTGAGA ATTCAGCTCC 961 TTAAAATTGG CAGTGCTATC CAGCGACTTC GCTGTGAATT AGACATTATG AATAAATGTA 1021 CTTCCCTTTG CTGTAAACAA TGTCAAGAAA CAGAAATAAC AACCAAAAAT GAAATATTCA 1081 GTTTATCCTT ATGTGGGCCG ATGGCAGCTT ATGTGAATCC TCATGGATAT GTGCATGAGA 1141 CACTTACTGT GTATAAGGCT TGCAACTTGA ATCTGATAGG CCGGCCTTCT ACAGAACACA 1201 GCTGGTTTCC TGGGTATGCC TGGACTGTTG CCCAGTGTAA GATCTGTGCA AGCCATATTG 1261 GATGGAAGTT TACGGCCACC AAAAAAGACA TGTCACCTCA AAAATTTTGG GGCTTAACGC 1321 GATCTGCTCT GTTGCCCACG ATCCCAGACA CTGAAGATGA AATAAGTCCA GACAAAGTAA 1381 TACTTTGCTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 1441 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 1501 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATAAC ACCCAATTTA CCAAAGATTT 1561 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt