Construct: ORF TRCN0000470667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013362.1_s317c1
- Derived from:
- ccsbBroadEn_01139
- DNA Barcode:
- AGTCTGAATACGAAAGTTATTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PA2G4 (5036)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5036 | PA2G4 | proliferation-associated 2G4 | NM_006191.3 | 100% | 100% | |
| 2 | human | 647033 | PA2G4P4 | proliferation-associated 2G... | NR_003284.1 | 41.8% | (many diffs) | |
| 3 | mouse | 18813 | Pa2g4 | proliferation-associated 2G4 | NM_011119.3 | 92.1% | 98.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1248
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gggcgaggac gagcaacagg agcaaactat cgctgaggac ctggtcgtga 121 ccaagtataa gatggggggc gacatcgcca acagggtact tcggtccttg gtggaagcat 181 ctagctcagg tgtgtcggta ctgagcctgt gtgagaaagg tgatgccatg attatggaag 241 aaacagggaa aatcttcaag aaagaaaagg aaatgaagaa aggtattgct tttcccacca 301 gcatttcggt aaataactgt gtatgtcact tctccccttt gaagagcgac caggattata 361 ttctcaagga aggtgacttg gtaaaaattg accttggggt ccatgtggat ggcttcatcg 421 ctaatgtagc tcacactttt gtggttgatg tagctcaggg gacccaagta acagggagga 481 aagcagatgt tattaaggca gctcaccttt gtgctgaagc tgccctacgc ctggtcaaac 541 ctggaaatca gaacacacaa gtgacagaag cctggaacaa agttgcccac tcatttaact 601 gcacgccaat agaaggtatg ctgtcacacc agttgaagca gcatgtcatc gatggagaaa 661 aaaccattat ccagaatccc acagaccagc agaagaagga ccatgaaaaa gctgaatttg 721 aggtacatga agtatatgct gtggatgttc tcgtcagctc aggagagggc aaggccaagg 781 atgcaggaca gagaaccact atttacaaac gagacccctc taaacagtat ggactgaaaa 841 tgaaaacttc acgtgCCTTC TTCAGTGAGG TGGAAAGGCG TTTTGATGCC ATGCCGTTTA 901 CTTTAAGAGC ATTTGAAGAT GAGAAGAAGG CTCGGATGGG TGTGGTGGAG TGCGCCAAAC 961 ATGAACTGCT GCAACCATTT AATGTTCTCT ATGAGAAGGA GGGTGAATTT GTTGCCCAGT 1021 TTAAATTTAC AGTTCTGCTC ATGCCCAATG GCCCCATGCG GATAACCAGT GGTCCCTTCG 1081 AGCCTGACCT CTACAAGTCT GAGATGGAGG TCCAGGATGC AGAGCTAAAG GCCCTCCTCC 1141 AGAGTTCTGC AAGTCGAAAA ACCCAGAAAA AGAAAAAAAA GAAGGCCTCC AAGACTGCAG 1201 AGAATGCCAC CAGTGGGGAA ACATTAGAAG AAAATGAAGC TGGGGACTGC CCAACTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGAAGTCTGA ATACGAAAGT TATTGTGACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt