Construct: ORF TRCN0000470688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008023.1_s317c1
- Derived from:
- ccsbBroadEn_10004
- DNA Barcode:
- AGGCCTCTAGCCCCACTGTTAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT79 (338785)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470688
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338785 | KRT79 | keratin 79 | NM_175834.3 | 99.8% | 99.8% | 242T>C;1116G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1671
- ORF length:
- 1605
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gtcctccgtc tctcggcaaa catactccac aaaagggggc ttcagctcca 121 actctgccag cggagggagt gggtcccagg cccgcaccag cttcagctca gtgacggtgt 181 ctcggagcag tggcagtggt ggcggggccc actgtggccc cggcacaggt ggctttggca 241 gccgaagcct ctataacttg gggggccaca agagtatctc tgtcagtgtg gccggagggg 301 ccttgtcggg gcgggctctg gggggctttg gctttggcag cagggcattt atgggacagg 361 gggctggcag gcagacgttt gggcctgctt gtcctcctgg ggggatccag gaggtcactg 421 tcaaccagag cctgctgacc cccctccatg tggagattga ccccgagatc cagcgagtgc 481 gcactcagga gcgggagcag atcaagaccc tcaacaacaa gttcgcctcc ttcatcgaca 541 aggtgcggtt cctggagcaa cagaataagg tgctggagac caagtgggca ctgctgcagg 601 agcagggcca gaacttgggt gtcaccagga acaacctgga gcccctcttt gaggcctacc 661 tgggtagcat gcggagcacg ctggacagac ttcagagcga gcgggggagg ctggactcag 721 agctcaggaa cgtgcaggac cttgtggagg acttcaagaa caagtacgag gatgaaatca 781 acaagcacac tgctgcagag aacgagtttg tggtgctcaa gaaggatgtg gatgcagcat 841 acatgggccg gatggatctg catggcaaag tgggcacctt gacccaggag attgacttcc 901 tgcagcaact ctatgaaatg gagctgagcc aagtgcagac ccacgtgtct aacaccaatg 961 tggtgctgtc catggacaac aaccgcaacc tggacctgga cagcatcatc gccgaggtca 1021 aggcccagta tgagctgatt gcccagagga gccgggctga ggccgaggcc tggtaccaga 1081 ccaagtatga ggagctgcag gtgactgctg ggaagcatgg ggacaacctg cgggacacca 1141 agaacgagat tgctgagctc acccgcacta tccagaggct tcagggggag gctgatgcag 1201 ccaagaagca gtgtcagcag ctgcagacgg ccattgcgga agcggagcag cgtggggagc 1261 tggcactcaa ggatgctcag aagaagcttg gggatctgga tgtggccctg caccaggcca 1321 aggaggacct gacacggctg ctgcgtgact accaggagct gatgaatgtc aagctggccc 1381 tggacgtgga gattgccacc taccgcaagc ttcTGGAGAG CGAGGAGAGC AGGATGTCTG 1441 GAGAATGTCC CAGTGCAGTC AGCATTTCTG TGACTGGCAA CTCCACCACT GTGTGCGGAG 1501 GTGGCGCAGC CAGCTTTGGA GGTGGCATCT CCCTGGGTGG GAGTGGGGGG GCCACCAAGG 1561 GTGGATTCAG CACAAATGTG GGCTATAGCA CCGTCAAGGG AGGGCCAGTC TCTGCGGGCA 1621 CCTCCATCCT GCGGAAGACC ACTACGGTCA AGACGTCCAG CCAGAGGTAT TACCCAACTT 1681 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1741 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1801 CTTGTGGAAA GGACGAAGGC CTCTAGCCCC ACTGTTAACC ACGCGTTAAG TCgacaatca 1861 acctctggat tacaaaattt gtgaaagatt