Construct: ORF TRCN0000470693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012947.1_s317c1
- Derived from:
- ccsbBroadEn_12138
- DNA Barcode:
- CGTTCTCCTTCAAAATTTGTACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PID1 (55022)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470693
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55022 | PID1 | phosphotyrosine interaction... | NM_001330157.2 | 100% | 100% | |
| 2 | human | 55022 | PID1 | phosphotyrosine interaction... | NM_001330158.2 | 81% | 78.3% | (many diffs) |
| 3 | human | 55022 | PID1 | phosphotyrosine interaction... | NM_001100818.2 | 77.4% | 77.4% | 29_175del |
| 4 | human | 55022 | PID1 | phosphotyrosine interaction... | NM_017933.5 | 66.4% | 64.1% | (many diffs) |
| 5 | human | 55022 | PID1 | phosphotyrosine interaction... | NM_001330156.1 | 65.9% | 63.6% | (many diffs) |
| 6 | human | 55022 | PID1 | phosphotyrosine interaction... | XM_017004404.1 | 54% | 52.1% | (many diffs) |
| 7 | mouse | 98496 | Pid1 | phosphotyrosine interaction... | XM_011238718.2 | 89% | 97% | (many diffs) |
| 8 | mouse | 98496 | Pid1 | phosphotyrosine interaction... | NM_001003948.2 | 68.9% | 75.1% | (many diffs) |
| 9 | mouse | 98496 | Pid1 | phosphotyrosine interaction... | XM_011238719.2 | 65.9% | 68.8% | (many diffs) |
| 10 | mouse | 98496 | Pid1 | phosphotyrosine interaction... | XM_017313047.1 | 65.9% | 68.8% | (many diffs) |
| 11 | mouse | 98496 | Pid1 | phosphotyrosine interaction... | XM_011238717.2 | 62.1% | 68.2% | (many diffs) |
| 12 | mouse | 98496 | Pid1 | phosphotyrosine interaction... | XM_017313045.1 | 61% | 63.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gcagccggcc acggagcgcc tgcaggttac ctacctgggc aaagtctcca 121 ccactggcat gcagtttttg tcaggctgca cagaaaagcc agtcattgag ctctggaaga 181 agcacacgct agcccgagag gatgtctttc cggccaatgc cctcctggaa aTCCGGCCAT 241 TCCAAGTTTG GCTCCATCAT CTCGACCACA AAGGGGAGGC CACAGTGCAC ATGGATACCT 301 TCCAGGTGGC CCGCATCGCC TACTGCACCG CCGACCACAA CGTGAGCCCC AACATCTTCG 361 CCTGGGTCTA CAGGGAGATC AATGATGACC TGTCCTACCA GATGGACTGC CACGCCGTGG 421 AGTGCGAGAG CAAGCTCGAG GCCAAGAAAC TGGCCCACGC CATGATGGAG GCCTTCAGGA 481 AGACTTTCCA CAGTATGAAG AGCGACGGGC GGATCCACAG CAACAGCTCC TCCGAAGAGG 541 TTTCCCAGGA ATTGGAATCC GATGATGGCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGTTC 721 TCCTTCAAAA TTTGTACAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt