Construct: ORF TRCN0000470709
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008755.1_s317c1
- Derived from:
- ccsbBroadEn_07269
- DNA Barcode:
- CTGTCTCGGGACTTGTCAGACGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEFF1 (8577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470709
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8577 | TMEFF1 | transmembrane protein with ... | NM_003692.5 | 99.9% | 100% | 105C>A |
| 2 | human | 100526694 | MSANTD3-TMEFF1 | MSANTD3-TMEFF1 readthrough | NM_001198812.1 | 76.6% | 68.6% | (many diffs) |
| 3 | mouse | 230157 | Tmeff1 | transmembrane protein with ... | NM_021436.2 | 86.6% | 93.6% | (many diffs) |
| 4 | mouse | 230157 | Tmeff1 | transmembrane protein with ... | XM_006537836.1 | 86.4% | 93.4% | (many diffs) |
| 5 | mouse | 230157 | Tmeff1 | transmembrane protein with ... | XM_006537838.1 | 86.3% | 93.1% | (many diffs) |
| 6 | mouse | 230157 | Tmeff1 | transmembrane protein with ... | XM_006537837.1 | 86% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgccgcagcc gctgaggcgc cgctccggct gcctgccgcg cctccgctcg 121 ccttctgctg ctacacgtcg gtgcttctgc tcttcgcctt ctctctgcca gggagccgcg 181 cgtccaacca gcccccgggt ggtggcggcg gcagcggcgg ggactgtccc ggcggcaaag 241 gcaagagcat caactgctca gaattaaatg tgagggagtc tgacgtaaga gtttgtgatg 301 agtcatcatg taaatatgga ggagtctgta aagaagatgg agatggtttg aaatgtgcat 361 gccaatttca gtgccataca aattatattc ctgtctgtgg atcaaatggg gacacttatc 421 aaaatgaatg ctttctcaga agggctgctt gtaagcacca gaaagagata acagtaatag 481 caagaggacc atgctactct gataatggat ctggatctgg agaaggagaa gaggaagggt 541 caggggcaga agttcacaga aaacactcca agtgtggacc ctgcaaatat aaagctgagt 601 gtgatgaaga tgcagaaaat gttgggtgtg tatgtaatat agattgcagt ggatacagtt 661 ttaatcctgt gtgtgcttct gatgggagtt cctataacaa tccctgtttt gttcgagaag 721 catcttgtat aaagcaagaa caaattgata taaggcaTCT TGGTCATTGC ACAGATACAG 781 ATGACACTAG TTTGTTGGGA AAGAAAGATG ATGGACTACA ATATCGACCA GATGTGAAAG 841 ATGCTAGTGA TCAAAGAGAA GATGTTTATA TTGGAAACCA CATGCCTTGC CCTGAAAACC 901 TCAATGGTTA CTGCATCCAT GGAAAATGTG AATTCATCTA TTCTACTCAG AAGGCTTCTT 961 GTAGATGTGA ATCTGGCTAC ACTGGACAGC ACTGTGAAAA GACAGACTTT AGTATTCTCT 1021 ATGTAGTGCC AAGTAGGCAA AAGCTCACTC ATGTTCTTAT TGCAGCAATT ATTGGAGCTG 1081 TACAGATTGC CATCATAGTA GCAATTGTAA TGTGCATAAC AAGAAAATGC CCCAAAAACA 1141 ATAGAGGACG TCGACAGAAG CAAAACCTAG GTCATTTTAC TTCAGATACG TCATCCAGAA 1201 TGGTTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGTCTCGG GACTTGTCAG ACGTTACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt