Construct: ORF TRCN0000470710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014755.1_s317c1
- Derived from:
- ccsbBroadEn_13300
- DNA Barcode:
- CGGCGGACAAGATCTGGAACAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM33 (155435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470710
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 155435 | RBM33 | RNA binding motif protein 33 | XM_011515840.1 | 22.2% | 21.4% | (many diffs) |
| 2 | human | 155435 | RBM33 | RNA binding motif protein 33 | NM_053043.3 | 21.9% | 21% | (many diffs) |
| 3 | human | 155435 | RBM33 | RNA binding motif protein 33 | XM_005249535.1 | 21.9% | 21% | (many diffs) |
| 4 | human | 155435 | RBM33 | RNA binding motif protein 33 | XM_005249534.1 | 21.7% | 20.8% | (many diffs) |
| 5 | human | 155435 | RBM33 | RNA binding motif protein 33 | XM_005249533.1 | 21.1% | 20.3% | (many diffs) |
| 6 | human | 155435 | RBM33 | RNA binding motif protein 33 | XM_005249532.1 | 21% | 20.2% | (many diffs) |
| 7 | human | 155435 | RBM33 | RNA binding motif protein 33 | XM_005249531.1 | 21% | 20.2% | (many diffs) |
| 8 | mouse | 381626 | Rbm33 | RNA binding motif protein 33 | XM_006535735.3 | 19.6% | 19.2% | (many diffs) |
| 9 | mouse | 381626 | Rbm33 | RNA binding motif protein 33 | XM_006535734.3 | 19.6% | 19.1% | (many diffs) |
| 10 | mouse | 381626 | Rbm33 | RNA binding motif protein 33 | XM_006535733.3 | 18.9% | 18.5% | (many diffs) |
| 11 | mouse | 381626 | Rbm33 | RNA binding motif protein 33 | NM_028234.1 | 18.9% | 18.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 870
- ORF length:
- 804
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccgccctg ggagcgagcg gaggagcagg cgccggagat gatgactttg 121 accagtttga taagcctggc gcggaacggt cgtggagaag aagagctgct gatgaggact 181 gggacagtga acttgaagat gatttacttg gagaagattt gctatctggc aaaaagaatc 241 agtcggattt gtcagatgaa gagctaaatg atgatctttt gcagagtgat aatgaagatg 301 aagaaaattt cagttctcag ggtgttacaa ttagtctgaa tgctacatct ggcatggtta 361 catcatttga actctctgac aacactaacg accaatctgg agaacaggaa tctgagtatg 421 aacaagaaca aggagaggat gaactggttt atcacaaatc TGATGGATCA GAATTGTATA 481 CTCAAGAGTA CCCAGAAGAA GGACAGTATG AAGGCCACGA AGCTGAGTTG ACAGAAGACC 541 AAATAGAATA TGTGGAAGAG CCAGAGGAGG AGCAGCTTTA CACTGATGAA GTGTTAGACA 601 TCGAGATCAA TGAACCTTTA GATGAATTTA CAGGAGGTAT GGAAACATTG GAACTTCAAA 661 AGGACATCAA AGAAGAATCA GATGAAGAAG AAGAAGATGA TGAAGAATCT GGACGATTAC 721 GTTTCAAAAC TGAAAGGAAA GAAGGAACAA TTATTAGGCT CTCAGATGTA ACTAGAGAGA 781 GAAGGAACAT TCCAGAAACT TTGGGTAACT TTTTTGCCTG TCTCCCATCC TCCTTTACTC 841 TCATTTCAAC TTCCTCCATA GTATTGCTGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 901 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 961 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGGCG 1021 GACAAGATCT GGAACAGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1081 tgaaagatt