Transcript: Human XM_005249533.1

PREDICTED: Homo sapiens RNA binding motif protein 33 (RBM33), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RBM33 (155435)
Length:
10463
CDS:
384..4019

Additional Resources:

NCBI RefSeq record:
XM_005249533.1
NBCI Gene record:
RBM33 (155435)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005249533.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148131 GAAAGCCATAGCTAAGTTCAA pLKO.1 3908 CDS 100% 4.950 6.930 N RBM33 n/a
2 TRCN0000230538 GAAGCTTAAAGGTCGTAAATA pLKO_005 6847 3UTR 100% 15.000 10.500 N RBM33 n/a
3 TRCN0000230536 GATGAGGAAACAAGGTTATAT pLKO_005 2853 CDS 100% 15.000 10.500 N RBM33 n/a
4 TRCN0000230537 CAGACTCAGCCGCTGCATAAA pLKO_005 3270 CDS 100% 13.200 9.240 N RBM33 n/a
5 TRCN0000218468 ATCGAGATCAATGAACCTTTA pLKO_005 918 CDS 100% 10.800 7.560 N RBM33 n/a
6 TRCN0000146529 GCAGAAATTCCACAGGCATAT pLKO.1 3956 CDS 100% 10.800 7.560 N RBM33 n/a
7 TRCN0000167857 GCATAATACAACTTCTCAGAA pLKO.1 2549 CDS 100% 4.950 3.465 N RBM33 n/a
8 TRCN0000150199 GCCACGTTTGTTAAATCCTTA pLKO.1 7010 3UTR 100% 4.950 3.465 N RBM33 n/a
9 TRCN0000148640 CGCATTAGCATTTCAGCAGAA pLKO.1 3941 CDS 100% 4.050 2.835 N RBM33 n/a
10 TRCN0000167649 GCTTAAAGATAGAAGAACAGA pLKO.1 2875 CDS 100% 3.000 2.100 N RBM33 n/a
11 TRCN0000149408 CAGAGTTTACAGATGCTTCCT pLKO.1 3879 CDS 100% 2.640 1.848 N RBM33 n/a
12 TRCN0000149980 CCTGAGCTATTTGAGCAAATA pLKO.1 5236 3UTR 100% 1.320 0.924 N RBM33 n/a
13 TRCN0000146861 CCACAGGCATATGATAGATTT pLKO.1 3965 CDS 100% 0.000 0.000 N RBM33 n/a
14 TRCN0000257117 CTCCAAGGTCAGGGTGATTAA pLKO_005 3449 CDS 100% 13.200 7.920 N RBM33 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005249533.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13300 pDONR223 100% 21.1% 20.3% None (many diffs) n/a
2 ccsbBroad304_13300 pLX_304 0% 21.1% 20.3% V5 (many diffs) n/a
3 TRCN0000470710 CGGCGGACAAGATCTGGAACAGTG pLX_317 50.9% 21.1% 20.3% V5 (many diffs) n/a
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