Construct: ORF TRCN0000470711
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015140.1_s317c1
- Derived from:
- ccsbBroadEn_09045
- DNA Barcode:
- AGAGGCCTGATGCCAAAGAAACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITIH5 (80760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470711
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80760 | ITIH5 | inter-alpha-trypsin inhibit... | NM_032817.6 | 99.8% | 99.8% | 135C>T;798C>T;2165G>A |
| 2 | human | 80760 | ITIH5 | inter-alpha-trypsin inhibit... | NM_030569.7 | 76.9% | 76.7% | (many diffs) |
| 3 | human | 80760 | ITIH5 | inter-alpha-trypsin inhibit... | XM_011519713.3 | 74.9% | 74.7% | (many diffs) |
| 4 | human | 80760 | ITIH5 | inter-alpha-trypsin inhibit... | NM_001001851.2 | 50.9% | 49% | (many diffs) |
| 5 | human | 80760 | ITIH5 | inter-alpha-trypsin inhibit... | XM_011519714.3 | 49.6% | 47.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2250
- ORF length:
- 2184
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gaactatgat tctgggcctc ccccatctac tgtcattaac caaaatgaaa 121 catttgccaa cataattttt aaacctactg tagtacaaca agccaggatt gcccagaatg 181 gaattttggg agactttatt attagatatg acgtcaatag agaacagagc attggggaca 241 tccaggttct aaatggctat tttgtgcact actttgctcc taaagacctt cctcctttac 301 ccaagaatgt ggtattcgtg cttgacagca gtgcttctat ggtgggaacc aaactccggc 361 agaccaagga tgccctcttc acaattctcc atgacctccg accccaggac cgtttcagta 421 tcattggatt ttccaaccgg atcaaagtat ggaaggacca cttgatatca gtcactccag 481 acagcatcag ggatgggaaa gtgtacattc accatatgtc acccactgga ggcacagaca 541 tcaacggggc cctgcagagg gccatcaggc tcctcaacaa gtacgtggcc cacagtggca 601 ttggagaccg gagcgtgtcc ctcatcgtct tcctgacgga tgggaagccc acggtcgggg 661 agacgcacac cctcaagatc ctcaacaaca cccgagaggc cgcccgaggc caagtctgca 721 tcttcaccat tggcatcggc aacgacgtgg acttcaggct gctggagaaa ctgtcgctgg 781 agaactgtgg cctcacacgg cgcgtgcacg aggaggagga cgcaggctcg cagctcatcg 841 ggttctacga tgaaatcagg actccgctcc tctctgacat ccgcatcgat tatcccccca 901 gctcagtggt gcaggccacc aagaccctgt tccccaacta cttcaacggc tcggagatca 961 tcattgcggg gaagctggtg gacaggaagc tggatcacct gcacgtggag gtcaccgcca 1021 gcaacagtaa gaaattcatc atcctgaaga cagatgtgcc tgtgcggcct cagaaggcag 1081 ggaaagatgt cacaggaagc cccaggcctg gaggcgatgg agagggggac accaaccaca 1141 tcgagcgtct ctggagctac ctcaccacaa aggagctgct gagctcctgg ctgcaaagtg 1201 acgatgaacc ggagaaggag cggctgcggc agcgggccca ggccctggct gtgagctacc 1261 gcttcctcac tcccttcacc tccatgaagc tgagggggcc ggtcccacgc atggatggcc 1321 tggaggaggc ccacggcatg tcggctgcca tgggacccga accggtggtg cagagcgtgc 1381 gaggagctgg cacgcagcca ggacctttgc tcaagaagcc ataccagcca agaattaaaa 1441 tctctaaaac atcagtggat ggtgatcccc actttgttgt ggatttcccc ctgagcagac 1501 tcaccgtgtg cttcaacatt gatgggcagc ccggggacat cctcaggctg gtctctgatc 1561 acagggactc tggtgtcaca gtgaacggag agttaattgg ggcacccgcc cctccaaatg 1621 gccacaagaa acagcgcact tacttgcgca ctatcaccat cctcatcaac aagccagaga 1681 gatcttatct cgagatcaca ccgagcagag tcatcttgga tggtggggac agactggtgc 1741 tcccctgcaa ccagagtgtg gtggtgggga gctgggggct ggaggtgtcc gtgtctgcca 1801 acgccaatgt caccgtcacc atccagggct ccatagcctt tgtcatcctc atccacctct 1861 acaaaaagcc ggcgcccTTC CAGCGACACC ACCTGGGTTT CTACATTGCC AACAGCGAGG 1921 GCCTTTCCAG CAACTGCCAC GGACTGCTGG GTCAGTTCCT GAATCAGGAT GCCAGACTCA 1981 CAGAAGACCC TGCAGGGCCC AGCCAGAACC TCACTCACCC TCTGCTCCTT CAGGTGGGAG 2041 AGGGGCCTGA GGCCGTCCTA ACAGTGAAAG GCCACCAAGT CCCAGTGGTC TGGAAGCAAA 2101 GGAAGATTTA CAACGGGGAA GAGCAGATAG ACTGCTGGTT TGCCAGGAAC AATGCCGCCA 2161 AACTGATTGA CGGGGAGTAC AAGGATTACC TGGCATCCCA TCCATTTGAC ACAGGGATGA 2221 CACTTGGCCA GGGAATGTCC AGGGAGCTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2281 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2341 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGAGG 2401 CCTGATGCCA AAGAAACCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2461 tgaaagatt