Construct: ORF TRCN0000470725
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006733.1_s317c1
- Derived from:
- ccsbBroadEn_01782
- DNA Barcode:
- AGGCGAACGTTATCGCCGATGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XG (7499)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470725
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 7499 | XG | Xg glycoprotein (Xg blood g... | NM_001141920.1 | 100% | 100% | |
2 | human | 7499 | XG | Xg glycoprotein (Xg blood g... | NM_175569.2 | 99.4% | 99.4% | 253_254insGTA |
3 | human | 7499 | XG | Xg glycoprotein (Xg blood g... | XM_005274587.4 | 92.3% | 92.3% | 376_420del |
4 | human | 7499 | XG | Xg glycoprotein (Xg blood g... | NM_001141919.1 | 91.8% | 91.8% | 253_254insGTA;373_417del |
5 | human | 7499 | XG | Xg glycoprotein (Xg blood g... | XM_017029787.2 | 85.2% | 85.2% | 60_61ins42;334_378del |
6 | human | 7499 | XG | Xg glycoprotein (Xg blood g... | XM_011545575.3 | 71.2% | 69% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 612
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagagctgg tggggacttc cctgtcttgc gttcctgtgt tttctaatgc 121 acgcccgagg TCAAAGAGAC TTTGATTTGG CAGATGCCCT TGATGACCCT GAACCCACCA 181 AGAAGCCAAA CTCAGATATC TACCCAAAGC CAAAACCACC TTACTACCCA CAGCCCGAGA 241 ATCCCGACAG CGGTGGAAAT ATCTACCCAA GGCCAAAGCC ACGCCCTCAA CCCCAGCCTG 301 GCAATTCCGG CAACAGTGGA GGTAGTTACT TCAATGATGT GGACCGTGAT GACGGACGCT 361 ACCCGCCCAG GCCCAGGCCA CGGCCGCCTG CAGGAGGTGG CGGCGGTGGC TACTCCAGTT 421 ATGGCAACTC CGACAACACG CACGGTGGAG ATCACCATTC AACGTATGGC AATCCAGAAG 481 GCAATATGGT AGCAAAAATC GTGTCTCCCA TCGTATCCGT GGTGGTGGTG ACACTGCTGG 541 GAGCAGCAGC CAGTTATTTC AAACTAAACA ATAGGAGAAA TTGTTTCAGG ACCCATGAAC 601 CAGAAAATGT CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGG CGAACGTTAT CGCCGATGAG 781 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t