Construct: ORF TRCN0000470746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010960.1_s317c1
- Derived from:
- ccsbBroadEn_02569
- DNA Barcode:
- TACCCCCTCCAAGTATCTCAAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTG3 (10950)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470746
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10950 | BTG3 | BTG anti-proliferation fact... | NM_001130914.2 | 100% | 100% | |
| 2 | human | 10950 | BTG3 | BTG anti-proliferation fact... | XM_024452052.1 | 100% | 100% | |
| 3 | human | 10950 | BTG3 | BTG anti-proliferation fact... | XM_011529441.1 | 96.7% | 96.7% | 1_30del |
| 4 | human | 10950 | BTG3 | BTG anti-proliferation fact... | XM_024452051.1 | 96.7% | 96.7% | 1_30del |
| 5 | human | 10950 | BTG3 | BTG anti-proliferation fact... | NM_006806.5 | 85.1% | 85.1% | 311_312ins132 |
| 6 | human | 10950 | BTG3 | BTG anti-proliferation fact... | XM_017028265.1 | 85.1% | 85.1% | 311_312ins132 |
| 7 | human | 10950 | BTG3 | BTG anti-proliferation fact... | XM_017028266.1 | 85.1% | 85.1% | 311_312ins132 |
| 8 | mouse | 12228 | Btg3 | B cell translocation gene 3 | NM_001297747.1 | 77.9% | 80% | (many diffs) |
| 9 | mouse | 12228 | Btg3 | B cell translocation gene 3 | NM_009770.3 | 77.9% | 80% | (many diffs) |
| 10 | mouse | 654432 | Gm7334 | predicted gene 7334; B-cell... | NR_002700.1 | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaatgaaatt gctgccgttg tcttcttttt cacaaggcta gttcgaaaac 121 atgataagtt gaaaaaagag gcagttgaga ggtttgctga gaaattgacc ctaatacttc 181 aagaaaaata taaaaatcac tggtatccag aaaaaccatc gaaaggacag gcctacagat 241 gtattcgtgt caataaattt cagagagttg atcctgatgt cctgaaagcc tgtgaaaaca 301 gctgcatctt gtatagtgac ctgggcttgc caaaggagct cactctctgg gtggacccat 361 gtgaggtgtg ctgtcgtaga gatggggttt caccatgttg gccagactgc tctcaaactc 421 ctgacctcgt gatccgcccg ccttggcctc ccaaagcgct ggattacagg cgtgagccac 481 tgcgcccggc ctcctccttt ttgattatgt atggagagaa aaacaatgca ttcattGTTG 541 CCAGCTTTGA AAATAAAGAT GAGAACAAGG ATGAGATCTC CAGGAAAGTT ACCAGGGCCC 601 TTGATAAGGT TACCTCTGAT TATCATTCAG GATCCTCTTC TTCAGATGAA GAAACAAGTA 661 AGGAAATGGA AGTGAAACCC AGTTCGGTGA CTGCAGCCGC AAGTCCTGTG TACCAGATTT 721 CAGAACTTAT ATTTCCACCT CTTCCAATGT GGCACCCTTT GCCCAGAAAA AAGCCAGGAA 781 TGTATCGAGG GAATGGCCAT CAGAATCACT ATCCTCCTCC TGTTCCATTT GGTTATCCAA 841 ATCAGGGAAG AAAAAATAAA CCATATCGCC CAATTCCAGT GACATGGGTA CCTCCTCCTG 901 GAATGCATTG TGACCGGAAT CACTGGATTA ATCCTCACAT GTTAGCACCT CACTACCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAT ACCCCCTCCA AGTATCTCAA ATCACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att