Construct: ORF TRCN0000470747
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014407.1_s317c1
- Derived from:
- ccsbBroadEn_15659
- DNA Barcode:
- ATTCCGCACTGTTCGGGGCTGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX7A2L (9167)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470747
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9167 | COX7A2L | cytochrome c oxidase subuni... | NM_001319036.1 | 99.7% | 100% | 21C>T |
| 2 | human | 9167 | COX7A2L | cytochrome c oxidase subuni... | NM_004718.4 | 99.7% | 100% | 21C>T |
| 3 | human | 9167 | COX7A2L | cytochrome c oxidase subuni... | NM_001319037.1 | 84.8% | 76.2% | (many diffs) |
| 4 | human | 9167 | COX7A2L | cytochrome c oxidase subuni... | NM_001319040.1 | 65.4% | 61.4% | 21C>T;202_203insAGAA;225_226ins113 |
| 5 | human | 9167 | COX7A2L | cytochrome c oxidase subuni... | NM_001319038.2 | 59.4% | 59.6% | (many diffs) |
| 6 | human | 9167 | COX7A2L | cytochrome c oxidase subuni... | NR_134947.1 | 25.9% | (many diffs) | |
| 7 | human | 9167 | COX7A2L | cytochrome c oxidase subuni... | NR_134948.1 | 16.2% | (many diffs) | |
| 8 | mouse | 20463 | Cox7a2l | cytochrome c oxidase subuni... | NM_009187.3 | 88.3% | 88.5% | (many diffs) |
| 9 | mouse | 20463 | Cox7a2l | cytochrome c oxidase subuni... | NM_001159529.1 | 73.4% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTA CTACAAGTTT AGTGGTTTCA CGCAGAAGTT GGCAGGAGCA TGGGCTTCGG 121 AGGCCTATAG CCCGCAGGGA TTAAAGCCTG TGGTTTCCAC AGAAGCACCA CCTATCATAT 181 TTGCCACACC AACTAAACTG ACCTCCGATT CCACAGTGTA TGATTATGCT GGGAAAAACA 241 AAGTTCCAGA GCTACAAAAG TTTTTCCAGA AAGCTGATGG TGTGCCCGTC TACCTGAAAC 301 GAGGCCTGCC TGACCAAATG CTTTACCGGA CCACCATGGC GCTGACTGTG GGAGGGACCA 361 TCTACTGCCT GATCGCCCTC TACATGGCTT CGCAGCCCAA AAACAAATGC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGAATTCCGC ACTGTTCGGG GCTGCGCACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt