Construct: ORF TRCN0000470750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004268.1_s317c1
- Derived from:
- ccsbBroadEn_01406
- DNA Barcode:
- ACTTGGCCGCACACAATTACATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNASE4 (6038)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 6038 | RNASE4 | ribonuclease A family member 4 | NM_001282192.2 | 100% | 100% | |
2 | human | 6038 | RNASE4 | ribonuclease A family member 4 | NM_001282193.2 | 100% | 100% | |
3 | human | 6038 | RNASE4 | ribonuclease A family member 4 | NM_002937.5 | 100% | 100% | |
4 | human | 6038 | RNASE4 | ribonuclease A family member 4 | NM_194431.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctgcagagg acccattcat tgcttctgct tttgctgctg accctgctgg 121 ggctggggct ggtccagccc tccTATGGCC AGGATGGCAT GTACCAGCGA TTCCTGCGGC 181 AACACGTGCA CCCTGAGGAG ACAGGTGGCA GTGATCGCTA CTGCAACTTG ATGATGCAAA 241 GACGGAAGAT GACTTTGTAT CACTGCAAGC GCTTCAACAC CTTCATCCAT GAAGATATCT 301 GGAACATTCG TAGTATCTGC AGCACCACCA ATATCCAATG CAAGAACGGC AAGATGAACT 361 GCCATGAGGG TGTAGTGAAG GTCACAGATT GCAGGGACAC AGGAAGTTCC AGGGCACCCA 421 ACTGCAGATA TCGGGCCATA GCGAGCACTA GACGTGTTGT CATTGCCTGT GAGGGTAACC 481 CACAGGTGCC TGTGCACTTT GACGGTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACTTGGCC 661 GCACACAATT ACATGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt