Construct: ORF TRCN0000470758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012998.1_s317c1
- Derived from:
- ccsbBroadEn_10368
- DNA Barcode:
- CGATTGACTCTCGGGAACACATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCZ1P-OR7E38P (389538)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470758
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389538 | CCZ1P-OR7E38P | CCZ1P-OR7E38P readthrough | NR_148008.1 | 29.7% | 1_353del;618_888del | |
| 2 | human | 389538 | CCZ1P-OR7E38P | CCZ1P-OR7E38P readthrough | NR_002822.4 | 28.2% | 1_401del;666_936del | |
| 3 | human | 26628 | OR7E47P | olfactory receptor family 7... | NR_120440.1 | 25.4% | (many diffs) | |
| 4 | human | 26628 | OR7E47P | olfactory receptor family 7... | NR_120439.1 | 24.8% | (many diffs) | |
| 5 | human | 26648 | OR7E24 | olfactory receptor family 7... | NM_001079935.1 | 23.8% | 20% | (many diffs) |
| 6 | human | 8587 | OR7E2P | olfactory receptor family 7... | NR_045004.1 | 22.3% | (many diffs) | |
| 7 | human | 10819 | OR7E14P | olfactory receptor family 7... | NR_045002.1 | 21.9% | (many diffs) | |
| 8 | human | 100506759 | OR7E37P | olfactory receptor family 7... | NR_002163.3 | 21.7% | (many diffs) | |
| 9 | human | 10821 | OR7E12P | olfactory receptor family 7... | NR_044999.1 | 21.5% | (many diffs) | |
| 10 | human | 79315 | OR7E91P | olfactory receptor family 7... | NR_002185.3 | 18% | (many diffs) | |
| 11 | human | 219445 | OR7E5P | olfactory receptor family 7... | NR_027688.2 | 12.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 113
- ORF end:
- 377
- ORF length:
- 264
- Sequence:
-
1 GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG 61 GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGTGCCT 121 GGTCACGGTG CTGGGGAACC TGCTCATCAT CCTGGCCATC AGCCCTGACT CCCACCTCCA 181 CACCCCCATG TACTTCTTCC TCTCCAACCT GTCCTTGCCT GACATCGGTT TCACCTCCAC 241 CACGGTCCCC AAGATGATTG TGGACATCCA GTCTCACAGC AGAGTCATCT CCTATGCAGG 301 CTGCCTGACT CAGATGTCTC TCTTTGCCAT TTTTGGAGGC ATGGAAGAGA GACATGCTCC 361 TGAGTGTGAT GGCCTATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 421 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 481 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGATTGAC TCTCGGGAAC 541 ACATTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt