Construct: ORF TRCN0000470759

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015560.1_s317c1
Derived from:
ccsbBroadEn_07265
DNA Barcode:
TACTATCTTTCACCGCGTTTTGTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MADD (8567)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470759
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8567 MADD MAP kinase activating death... NM_001135943.2 99.8% 99.8% (many diffs)
2 human 8567 MADD MAP kinase activating death... NM_130472.3 99.8% 99.7% (many diffs)
3 human 8567 MADD MAP kinase activating death... XM_005253203.3 99.8% 99.7% (many diffs)
4 human 8567 MADD MAP kinase activating death... NM_001135944.2 99.6% 99.6% (many diffs)
5 human 8567 MADD MAP kinase activating death... XM_005253204.3 99.6% 99.6% (many diffs)
6 human 8567 MADD MAP kinase activating death... NM_130471.3 98.5% 98.4% (many diffs)
7 human 8567 MADD MAP kinase activating death... XM_005253201.3 98.5% 98.4% (many diffs)
8 human 8567 MADD MAP kinase activating death... XM_017018487.2 97.2% 97% (many diffs)
9 human 8567 MADD MAP kinase activating death... NM_130476.3 97.1% 97.1% (many diffs)
10 human 8567 MADD MAP kinase activating death... NM_130470.3 97.1% 97% (many diffs)
11 human 8567 MADD MAP kinase activating death... XM_005253199.3 97.1% 97% (many diffs)
12 human 8567 MADD MAP kinase activating death... XM_017018486.2 97% 97% (many diffs)
13 human 8567 MADD MAP kinase activating death... XM_017018484.2 96.9% 96.9% (many diffs)
14 human 8567 MADD MAP kinase activating death... XM_017018485.2 96.9% 96.9% (many diffs)
15 human 8567 MADD MAP kinase activating death... XM_024448749.1 96% 95.8% (many diffs)
16 human 8567 MADD MAP kinase activating death... XM_017018483.2 96% 95.8% (many diffs)
17 human 8567 MADD MAP kinase activating death... XM_017018480.2 95.9% 95.8% (many diffs)
18 human 8567 MADD MAP kinase activating death... XM_024448748.1 95.9% 95.8% (many diffs)
19 human 8567 MADD MAP kinase activating death... NM_130473.3 95.8% 95.8% (many diffs)
20 human 8567 MADD MAP kinase activating death... XM_005253196.3 95.8% 95.8% (many diffs)
21 human 8567 MADD MAP kinase activating death... XM_024448750.1 95.8% 95.7% (many diffs)
22 human 8567 MADD MAP kinase activating death... XM_017018482.2 95.7% 95.7% (many diffs)
23 human 8567 MADD MAP kinase activating death... XM_017018481.1 95.7% 95.6% (many diffs)
24 human 8567 MADD MAP kinase activating death... NM_130474.3 95.5% 95.2% (many diffs)
25 human 8567 MADD MAP kinase activating death... XM_005253205.2 95.5% 95.2% (many diffs)
26 human 8567 MADD MAP kinase activating death... XM_017018479.2 94.5% 94.4% (many diffs)
27 human 8567 MADD MAP kinase activating death... NM_003682.4 93.6% 93.5% (many diffs)
28 human 8567 MADD MAP kinase activating death... XM_005253189.3 93.6% 93.5% (many diffs)
29 human 8567 MADD MAP kinase activating death... XM_024448746.1 93.5% 93.3% (many diffs)
30 human 8567 MADD MAP kinase activating death... XM_017018478.2 93.4% 93.3% (many diffs)
31 human 8567 MADD MAP kinase activating death... XM_024448745.1 93.4% 93.3% (many diffs)
32 human 8567 MADD MAP kinase activating death... XM_024448747.1 93.3% 93.2% (many diffs)
33 human 8567 MADD MAP kinase activating death... XM_017018507.1 93% 92.7% (many diffs)
34 human 8567 MADD MAP kinase activating death... XM_017018500.1 93% 92.7% (many diffs)
35 human 8567 MADD MAP kinase activating death... XM_017018505.2 92.9% 92.6% (many diffs)
36 human 8567 MADD MAP kinase activating death... XM_017018506.1 92.9% 92.6% (many diffs)
37 human 8567 MADD MAP kinase activating death... XM_024448755.1 92% 91.7% (many diffs)
38 human 8567 MADD MAP kinase activating death... XM_024448753.1 92% 91.7% (many diffs)
39 human 8567 MADD MAP kinase activating death... XM_024448754.1 92% 91.7% (many diffs)
40 human 8567 MADD MAP kinase activating death... XM_017018498.1 91.8% 91.5% (many diffs)
41 human 8567 MADD MAP kinase activating death... XM_017018499.1 91.8% 91.5% (many diffs)
42 human 8567 MADD MAP kinase activating death... XM_024448752.1 90.7% 90.4% (many diffs)
43 human 8567 MADD MAP kinase activating death... XM_024448751.1 90.7% 90.4% (many diffs)
44 human 8567 MADD MAP kinase activating death... XM_017018491.2 90.6% 90.4% (many diffs)
45 human 8567 MADD MAP kinase activating death... XM_017018490.1 90.6% 90.3% (many diffs)
46 human 8567 MADD MAP kinase activating death... XM_017018493.1 90.5% 90.2% (many diffs)
47 human 8567 MADD MAP kinase activating death... XM_017018492.1 90.4% 90.2% (many diffs)
48 human 8567 MADD MAP kinase activating death... XM_005253200.2 89.6% 89.3% (many diffs)
49 human 8567 MADD MAP kinase activating death... NM_130475.3 89.6% 89.3% (many diffs)
50 human 8567 MADD MAP kinase activating death... XM_017018488.1 89.4% 89.1% (many diffs)
51 human 8567 MADD MAP kinase activating death... XM_017018489.1 89.4% 89.1% (many diffs)
52 mouse 228355 Madd MAP-kinase activating death... XM_017317630.1 89.2% 95.5% (many diffs)
53 mouse 228355 Madd MAP-kinase activating death... XM_017317629.1 89.1% 95.5% (many diffs)
54 mouse 228355 Madd MAP-kinase activating death... XM_017317632.1 89% 95.4% (many diffs)
55 mouse 228355 Madd MAP-kinase activating death... XM_017317627.1 88% 94.3% (many diffs)
56 mouse 228355 Madd MAP-kinase activating death... XM_017317624.1 86.8% 93% (many diffs)
57 mouse 228355 Madd MAP-kinase activating death... XM_017317623.1 86.7% 92.9% (many diffs)
58 mouse 228355 Madd MAP-kinase activating death... XM_017317621.1 85.6% 91.7% (many diffs)
59 mouse 228355 Madd MAP-kinase activating death... XM_017317634.1 85.2% 91.1% (many diffs)
60 mouse 228355 Madd MAP-kinase activating death... NM_001177720.1 84.5% 90.4% (many diffs)
61 mouse 228355 Madd MAP-kinase activating death... XM_017317620.1 84.5% 90.4% (many diffs)
62 mouse 228355 Madd MAP-kinase activating death... XM_017317619.1 83.6% 89.5% (many diffs)
63 mouse 228355 Madd MAP-kinase activating death... NM_145527.4 80.9% 86.4% (many diffs)
64 mouse 228355 Madd MAP-kinase activating death... XM_017317628.1 80.9% 86.4% (many diffs)
65 mouse 228355 Madd MAP-kinase activating death... NM_001177719.1 80% 85.4% (many diffs)
66 mouse 228355 Madd MAP-kinase activating death... XM_017317626.1 80% 85.4% (many diffs)
67 mouse 228355 Madd MAP-kinase activating death... XM_017317625.1 79.9% 85.4% (many diffs)
68 mouse 228355 Madd MAP-kinase activating death... XR_001782107.1 48.5% (many diffs)
69 mouse 228355 Madd MAP-kinase activating death... XR_001782105.1 47.5% (many diffs)
70 mouse 228355 Madd MAP-kinase activating death... XR_001782106.1 47.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
4698
ORF length:
4632
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gcaaaagaag aagttctgtc ctcggttact tgactatcta gtgatcgtag
121gggccaggca cccgagcagt gatagcgtgg cccagactcc tgaattgcta cggcgatacc
181ccttggagga tcacactgag tttcccctgc ccccagatgt agtgttcttc tgccagcccg
241agggctgcct gagcgtgcgg cagcggcgca tgagccttcg ggatgatacc tcttttgtct
301tcaccctcac tgacaaggac actggagtca cgcgatatgg catctgtgtt aacttctacc
361gctccttcca aaagcgaatc tctaaggaga agggggaagg tggggcaggg tcccgtggga
421aggaaggaac ccatgccacc tgtgcctcag aagagggtgg cactgagagc tcagagagtg
481gctcatccct gcagcctctc agtgctgact ctacccctga tgtgaaccag tctcctcggg
541gcaaacgccg ggccaaggcg gggagccgct cccgcaacag tactctcacg tccctgtgcg
601tgctcagcca ctaccctttc ttctccacct tccgagagtg tttgtatact ctcaagcgcc
661tggtggactg ctgtagtgag cgccttctgg gcaagaaact gggcatccct cgaggcgtac
721aaagggacac catgtggcgg atctttactg gatcgctgct ggtagaggag aagtcaagtg
781cccttctgca tgaccttcga gagattgagg cctggatcta tcgattgctg cgctccccag
841tacccgtctc tgggcagaag cgagtagaca tcgaggtcct accccaagag ctccagccag
901ctctgacctt tgctcttcca gacccatctc gattcaccct agtggatttc ccactgcacc
961ttcccttgga acttctaggt gtggacgcct gtctccaggt gctaacctgc attctgttag
1021agcacaaggt ggtgctacag tcccgagact acaatgcact ctccatgtct gtgatggcat
1081tcgtggcaat gatctaccca ctggaatata tgtttcctgt catcccgctg ctacccacct
1141gcatggcatc agcagagcag ctgctgttgg ctccaacccc gtacatcatt ggggttcctg
1201ccagcttctt cctctacaaa ctggacttca aaatgcctga tgatgtatgg ctagtggatc
1261tggacagcaa tagggtgatt gcccccacca atgcagaagt gctgcctatc ctgccagaac
1321cagaatcact agagctgaaa aagcatttaa agcaggcctt ggccagcatg agtctcaaca
1381cccagcccat cctcaatctg gagaaatttc atgagggcca ggagatcccc cttctcttgg
1441gaaggccttc taatgacctg cagtccacac cgtccactga attcaaccca ctcatctatg
1501gcaatgatgt ggattctgtg gatgttgcaa ccagggttgc catggtacgg ttcttcaatt
1561ccgccaacgt gctgcaggga tttcagatgc acacgcgtac cctgcgcctc tttcctcggc
1621ctgtggtagc ttttcaagct ggctcctttc tagcctcacg tccccggcag actccttttg
1681ccgagaaatt ggccaggact caggctgtgg agtactttgg ggaatggatc cttaacccca
1741ccaactatgc ctttcagcga attcacaaca atatgtttga tccagccctg attggtgaca
1801agccaaagtg gtatgctcat cagctgcagc ctatccacta tcgcgtctat gacagcaatt
1861cccagctggc tgaggccctg agtgtaccac cagagcggga ctctgactcc gaacctactg
1921atgatagtgg cagtgatagt atggattatg acgattcaag ctcttcttac tcctcccttg
1981gtgactttgt cagtgaaatg atgaaatgtg acattaatgg tgatactccc aatgtggacc
2041ctctgacaca tgcagcactg ggggatgcca gcgaggtgga gattgacgag ctgcagaatc
2101agaaggaagc agaagagcct ggcccagaca gtgagaactc tcaggaaaac accccactgc
2161gctccagctc tagcaccaca gccagcagca gccccagcac tgtcatccac ggagccaact
2221ctgaacctgc tgactctacg gagatggatg ataaggcagc agtaggcgtc tccaagcccc
2281tcccttccgt gcctcccagc attggcaaat cgaacgtgga cagacgtcag gcagaaattg
2341gagagggggc tcaaaagctg ctgcggccca acagcttgag actggcaagt gactcagatg
2401cagagtcaga ctctcgggca agctctccca actccaccgt ctccaacacc agcaccgagg
2461gcttcggggg catcatgtct tttgccagca gcctctatcg gaaccacagt accagcttca
2521gtctttcaaa cctcacactg cccaccaaag gtgcccgaga gaaggccacg cccttcccca
2581gtctgaaagg aaacaggagg gcgttagtgg atcagaagtc atctgtcatt aaacacagcc
2641caacagtgaa aagagaacct ccatcacccc agggtcgatc cagcaattct agtgagaacc
2701agcagttcct gaaggaggtg gtgcacagcg tgctggacgg ccagggagtt ggctggctca
2761acatgaaaaa ggtgcgcggg ctgctggaga gcgagcagct gcgagtcttt gtcctgagca
2821agctgaaccg catggtgcag tcagaggacg atgcccggca ggacatcatc ccggatgtgg
2881agatcagtcg gaaggtgtac aagggaatgt tagacctcct caagtgtaca gtcctcagct
2941tggagcagtc ctatgcccac gcgggtctgg gtggcatggc cagcatcttt gggtttttgg
3001agattgccca gacccactac tatagtaaag aaccagacaa gcggaagaga agtccaacag
3061aaagtgtaaa taccccagtt ggcaaggatc ctggcctagc tgggcggggg gacccaaagg
3121ctatggcaca actgagagtt ccacaactgg gacctcgggc accaagtgcc acaggaaagg
3181gtcctaagga actggacacc agaagtttaa aggaagaaaa ttttatagca tctattgggc
3241ctgaagtaat caaacctgtc tttgaccttg gtgagacaga ggagaaaaag tcccagatca
3301gcgcagacag tggtgtgagc ctgacgtcta gttcccagag gactgatcaa gactctgtca
3361tcggcgtgag tccagctgtt atgatccgca gctcaagtca ggattctgaa gttagcaccg
3421tgagtaatag ctctggagag acccttggag ctgacagtga cttgagcagc aatgcaggtg
3481atggaccagg tggcgagggc agtgttcacc tggcaagctc tcggggcact ttgtctgata
3541gtgaaattga gaccaactct gccacaagca ctatctttgg taaagcccac agcttgaagc
3601caagcataaa ggagaagctg gcaggcagcc ccattcgtac ttctgaagat gtgagccagc
3661gagtctatct ctatgaggga ctcctaggca aagagcgttc tactttatgg gaccaaatgc
3721aattctggga agatgccttc ttagatgctg tgatgttgga gagagaaggg atgggtatgg
3781accagggtcc ccaggaaatg atcgacaggt acctgtccct tggagaacat gaccggaagc
3841gcctggaaga tgatgaagat cgcttgctgg ccacacttct gcacaacctc atctcctaca
3901tgctgctgat gaaggtaaat aagaatgaca tccgcaagaa ggtgaggcgc ctaatgggaa
3961agtcgcacat tgggcttgtg tacagccagc aaatcaatga ggtgcttgat cagctggcga
4021acctgaatgg acgcgatctc tctatctggt ccagtggcag ccggcacatg aagaagcaga
4081catttgtggt acatgcaggg acagatacaa acggagatat ctttttcatg gaggtgtgcg
4141atgactgtgt ggtgttgcgt agtaacatcg gaacagtgta tgagcgctgg tggtacgaga
4201agctcatcaa catgacctac tgtcccaaga cGAAGGTGTT GTGCTTGTGG CGTAGAAATG
4261GCTCTGAGAC CCAGCTCAAC AAGTTCTATA CTAAAAAGTG TCGGGAGCTG TACTACTGTG
4321TGAAGGACAG CATGGAGCGC GCTGCCGCCC GACAGCAAAG CATCAAACCC GGACCTGAAT
4381TGGGTGGCGA GTTCCCTGTG CAGGACCTGA AGACTGGTGA GGGTGGCCTG CTGCAGGTGA
4441CCCTGGAAGG GATCAACCTC AAATTCATGC ACAATCAGGT TTTCATAGAG CTGAATCACA
4501TTAAAAAGTG CAATACAGTT CGAGGCGTCT TTGTCCTGGA GGAATTTGTT CCTGAAATTA
4561AAGAAGTGGT GAGCCACAAG TACAAGACAC CAATGGCCCA CGAAATCTGC TACTCCGTAT
4621TATGTCTCTT CTCGTACGTG GCTGCAGTTC ATAGCAGTGA GGAAGATCTC AGAACCCCGC
4681CCCGGCCTGT CTCTAGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
4741TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
4801GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACTATC TTTCACCGCG
4861TTTTGTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)