Construct: ORF TRCN0000470771
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010144.1_s317c1
- Derived from:
- ccsbBroadEn_12230
- DNA Barcode:
- TGATGGTGATACCCCTCCATTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX60 (55601)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470771
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55601 | DDX60 | DExD/H-box helicase 60 | NM_017631.6 | 10.6% | 10.6% | 1_4587del |
| 2 | human | 55601 | DDX60 | DExD/H-box helicase 60 | XM_024454132.1 | 10.6% | 10.6% | 1_4587del |
| 3 | human | 55601 | DDX60 | DExD/H-box helicase 60 | XM_024454133.1 | 10.6% | 10.6% | 1_4587del |
| 4 | human | 55601 | DDX60 | DExD/H-box helicase 60 | XM_011532103.3 | 8.8% | 8.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 615
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aattatggag gactttacca ctttcctacg aattgtttcc aaactggctg 121 atatgaatca ggaatatcaa ctcccattgt caaaaatcaa attcacaggt aaagaatgtg 181 aagactctca actcgtatct catttgatga gctgcaagga aggaagagta gcaatttcac 241 catttgtttG TCTGTCTGGG AACTTTGATG ATGATTTGCT TCGACTAGAA ACTCCAAACC 301 ATGTTACTCT AGGCACAATC GGTGTCAATC GCTCTCAGGC TCCAGTGCTG TTGTCACAGA 361 AATTTGATAA CCGAGGAAGG AAAATGTCGC TTAATGCCTA TGCACTGGAT TTCTACAAAC 421 ATGGTTCCTT GATAGGATTA GTCCAGGATA ACAGGATGAA TGAAGGAGAT GCTTATTATT 481 TGTTGAAGGA TTTTGCACTC ACCATTAAAT CTATCAGTGT TTCCTTGCGT GAGCTATGTG 541 AAAATGAAGA CGACAACGTT GTCTTAGCCT TTGAACAACT GAGTACAACT TTTTGGGAAA 601 AGTTAAACAA AGTCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGATGGTGAT ACCCCTCCAT 781 TAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt