Construct: ORF TRCN0000470777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011027.1_s317c1
- Derived from:
- ccsbBroadEn_10264
- DNA Barcode:
- GTACTCACCACGTTGCAGTTCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD14C (84278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84278 | MFSD14C | major facilitator superfami... | NM_001355228.1 | 56.6% | 56.6% | 1_195del |
| 2 | human | 84278 | MFSD14C | major facilitator superfami... | XM_024447698.1 | 49.5% | 42.5% | (many diffs) |
| 3 | human | 84278 | MFSD14C | major facilitator superfami... | NM_001355230.1 | 47.1% | 45.3% | (many diffs) |
| 4 | human | 84278 | MFSD14C | major facilitator superfami... | XM_024447700.1 | 46% | 44.6% | (many diffs) |
| 5 | human | 84278 | MFSD14C | major facilitator superfami... | XM_024447699.1 | 45.9% | 42.4% | (many diffs) |
| 6 | human | 84278 | MFSD14C | major facilitator superfami... | NM_001355229.2 | 44.1% | 43.5% | (many diffs) |
| 7 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015221.1 | 26.1% | 21.6% | (many diffs) |
| 8 | human | 84641 | MFSD14B | major facilitator superfami... | XM_011519114.2 | 18.4% | 15.3% | (many diffs) |
| 9 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015217.2 | 18.4% | 15.3% | (many diffs) |
| 10 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015218.1 | 18.4% | 15.3% | (many diffs) |
| 11 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015219.2 | 18.3% | 15.5% | (many diffs) |
| 12 | human | 84641 | MFSD14B | major facilitator superfami... | NM_032558.3 | 16% | 13.3% | (many diffs) |
| 13 | human | 84278 | MFSD14C | major facilitator superfami... | XR_002956812.1 | 12.3% | (many diffs) | |
| 14 | human | 84641 | MFSD14B | major facilitator superfami... | XR_002956813.1 | 7.6% | (many diffs) | |
| 15 | mouse | 66631 | Mfsd14b | major facilitator superfami... | NM_001083901.1 | 14.3% | 12.9% | (many diffs) |
| 16 | mouse | 66631 | Mfsd14b | major facilitator superfami... | NM_133680.3 | 14.3% | 12.9% | (many diffs) |
| 17 | mouse | 66631 | Mfsd14b | major facilitator superfami... | XM_011244550.2 | 14.3% | 12.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 179
- ORF end:
- 434
- ORF length:
- 255
- Sequence:
-
1 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT 61 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 121 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT 181 GTTGACTGTT CTACATGAAA CATTTTCTCA ACACACATTC CTCATGAATG GTCTCATTCA 241 AGGTGTAAAG GGCCTGCTCT CTTTTTTGAG TGCCCCACTC ATTGGTGCCC TGTCTGATGT 301 GTGGGGGAGG AAGCCCTTTC TCCTCGGCAC TGTATTCTTT ACCTGCTTCC CAATCCCACT 361 GATGAGGATC AGCCCATGCC GGGTGTGGTG GCGTGCACCT GTAGTCCCAG CTACCTGTGG 421 CAGGAGAATG GCTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 481 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 541 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TACTCACCAC GTTGCAGTTC 601 AGGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att