Construct: ORF TRCN0000470818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008903.1_s317c1
- Derived from:
- ccsbBroadEn_10476
- DNA Barcode:
- GATCGAAGCCCTATTGCATAAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRRC1 (133619)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470818
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133619 | PRRC1 | proline rich coiled-coil 1 | NM_130809.5 | 99.7% | 99.7% | 1_3delATG;1299G>A |
| 2 | human | 133619 | PRRC1 | proline rich coiled-coil 1 | XM_006714529.3 | 99.7% | 99.7% | 1_3delATG;1299G>A |
| 3 | human | 133619 | PRRC1 | proline rich coiled-coil 1 | NM_001286808.2 | 93.9% | 94.1% | (many diffs) |
| 4 | mouse | 73137 | Prrc1 | proline-rich coiled-coil 1 | NM_028447.3 | 80.9% | 87.1% | (many diffs) |
| 5 | mouse | 73137 | Prrc1 | proline-rich coiled-coil 1 | XM_006526287.3 | 80.9% | 87.1% | (many diffs) |
| 6 | mouse | 73137 | Prrc1 | proline-rich coiled-coil 1 | XM_006526288.2 | 80.9% | 87.1% | (many diffs) |
| 7 | mouse | 73137 | Prrc1 | proline-rich coiled-coil 1 | XM_006526289.2 | 80.9% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agagagtgga atagagacaa caccacctgg gactcctcca ccaaatcctg 121 cagggctggc tgctactgct atgtcttcta cccctgttcc attagcggca accagttctt 181 tttcttctcc aaatgtatcc tccatggagt ccttcccacc actcgcatac tctactcctc 241 agccgcccct tcctcctgtg aggccttcag caccattacc ttttgtgcct cctcctgcag 301 ttccttctgt cccaccactt gttacttcta tgccacctcc tgtttctcca tcaactgctg 361 ctgccttcgg taatcctcct gtatctcact tcccaccttc aacttctgcc ccaaacactc 421 ttttacctgc acccccttcg ggtcctccta tatcaggatt ttctgttggt tcaacttatg 481 acattacaag gggacatgct gggagagctc cccagacacc cctgatgcca tcattttctg 541 caccttcagg aacaggtctt ttgccaactc ctattactca gcaagccagt ttgacatctc 601 tggcacaggg aactggaacc acatcagcca ttactttccc agaggagcaa gaagacccta 661 gaattactag aggtcaggat gaagcatctg ctggtggaat ctggggtttt attaagggtg 721 tggctgggaa tcctatggtg aagtctgtgc ttgataagac aaaacattca gtagaaagca 781 tgattacaac gctggaccct ggcatggctc cctatatcaa atctggaggt gaactggata 841 ttgtagtgac ctcaaataaa gaagtaaaag ttgctgctgt ccgagatgcc ttccaggagg 901 tctttggctt agctgtggtt gtaggggaag ctggacagtc caatattgcc ccacaaccag 961 tgggctatgc agctggatta aaaggtgctc aggaacggat agatagctTG CGTCGAACTG 1021 GGGTGATCCA TGAAAAACAG ACAGCTGTGT CAGTAGAAAA CTTCATTGCA GAATTGCTGC 1081 CTGACAAATG GTTTGACATT GGTTGTTTGG TGGTTGAAGA TCCTGTCCAT GGCATTCATC 1141 TAGAAACATT TACACAAGCC ACACCAGTGC CTTTGGAATT TGTACAGCAG GCTCAAAGTC 1201 TAACTCCCCA GGACTATAAT CTGAGGTGGT CAGGCCTTTT GGTGACAGTG GGTGAAGTCC 1261 TGGAAAAGAG TTTACTGAAT GTCAGCCGGA CTGATTGGCA CATGGCATTT ACTGGGATGT 1321 CCCGTCGGCA GATGATCTAC AGTGCAGCCA GAGCGATAGC AGGCATGTAT AAACAGCGCC 1381 TGCCACCCAG GACAGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGATCGAA GCCCTATTGC 1561 ATAAACAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt