Construct: ORF TRCN0000470819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007082.1_s317c1
- Derived from:
- ccsbBroadEn_06130
- DNA Barcode:
- CGATCACTGAATGGTGGTAGCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP4 (1846)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1846 | DUSP4 | dual specificity phosphatase 4 | NM_001394.7 | 99.9% | 100% | 522T>C |
| 2 | human | 1846 | DUSP4 | dual specificity phosphatase 4 | NM_057158.3 | 68.4% | 65.1% | (many diffs) |
| 3 | human | 1846 | DUSP4 | dual specificity phosphatase 4 | XM_011544428.3 | 68.4% | 65.1% | (many diffs) |
| 4 | mouse | 319520 | Dusp4 | dual specificity phosphatase 4 | NM_176933.4 | 89% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1248
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gacgatggag gagctgcggg agatggactg cagtgtgctc aaaaggctga 121 tgaaccggga cgagaatggc ggcggcgcgg gcggcagcgg cagccacggc accctggggc 181 tgccgagcgg cggcaagtgc ctgctgctgg actgcagacc gttcctggcg cacagcgcgg 241 gctacatcct aggttcggtc aacgtgcgct gtaacaccat cgtgcggcgg cgggctaagg 301 gctccgtgag cctggagcag atcctgcccg ccgaggagga ggtacgcgcc cgcttgcgct 361 ccggcctcta ctcggcggtc atcgtctacg acgagcgcag cccgcgcgcc gagagcctcc 421 gcgaggacag caccgtgtcg ctggtggtgc aggcgctgcg ccgcaacgcc gagcgcaccg 481 acatctgcct gctcaaaggc ggctatgaga ggttttcctc cgagtaccca gaattctgtt 541 ctaaaaccaa ggccctggca gccatcccac ccccggttcc ccccagcgcc acagagccct 601 tggacctggg ctgcagctcc tgtgggaccc cactacacga ccaggggggt cctgtggaga 661 tccttccctt cctctacctc ggcagtgcct accatgctgc ccggagagac atgctggacg 721 ccctgggcat cacggctctg ttgaatgtct ccTCGGACTG CCCAAACCAC TTTGAAGGAC 781 ACTATCAGTA CAAGTGCATC CCAGTGGAAG ATAACCACAA GGCCGACATC AGCTCCTGGT 841 TCATGGAAGC CATAGAGTAC ATCGATGCCG TGAAGGACTG CCGTGGGCGC GTGCTGGTGC 901 ACTGCCAGGC GGGCATCTCG CGGTCGGCCA CCATCTGCCT GGCCTACCTG ATGATGAAGA 961 AACGGGTGAG GCTGGAGGAG GCCTTCGAGT TCGTTAAGCA GCGCCGCAGC ATCATCTCGC 1021 CCAACTTCAG CTTCATGGGG CAGCTGCTGC AGTTCGAGTC CCAGGTGCTG GCCACGTCCT 1081 GTGCTGCGGA GGCTGCTAGC CCCTCGGGAC CCCTGCGGGA GCGGGGCAAG ACCCCCGCCA 1141 CCCCCACCTC GCAGTTCGTC TTCAGCTTTC CGGTCTCCGT GGGCGTGCAC TCGGCCCCCA 1201 GCAGCCTGCC CTACCTGCAC AGCCCCATCA CCACCTCTCC CAGCTGTTAC CCAACTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGACGATCAC TGAATGGTGG TAGCCGTACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt