Construct: ORF TRCN0000470848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009284.3_s317c1
- Derived from:
- ccsbBroadEn_01929
- DNA Barcode:
- ACAAACGGTCTTTGCTTCCTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYRK2 (8445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470848
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_006482.3 | 100% | 100% | |
| 2 | human | 8445 | DYRK2 | dual specificity tyrosine p... | NM_003583.4 | 87.8% | 87.8% | 0_1ins219 |
| 3 | human | 8445 | DYRK2 | dual specificity tyrosine p... | XM_017020032.1 | 87.8% | 87.8% | 0_1ins219 |
| 4 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | NM_001014390.2 | 90.4% | 97.1% | (many diffs) |
| 5 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_006514046.3 | 80.4% | 86.2% | (many diffs) |
| 6 | mouse | 69181 | Dyrk2 | dual-specificity tyrosine-(... | XM_011243552.2 | 79.1% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1869
- ORF length:
- 1803
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt aaccaggaaa ccttcggccg ccgctcccgc cgcctacccg accggccgag 121 gtggggacag cgccgttcgt cagcttcagg cttccccggg gctcggtgca ggggccaccc 181 ggagcggagt ggggactggc ccgccctccc ccatcgccct gccgcctctc cgggccagca 241 acgctgccgc cgcagcccac acgattggcg gcagtaagca cacaatgaat gatcacctgc 301 atgtcggcag ccacgctcac ggacagatcc aggttcaaca gttgtttgag gataacagta 361 acaagcggac agtgctcacg acacaaccaa atgggcttac aacagtgggc aaaacgggct 421 tgccagtggt gccagagcgg cagctggaca gcattcatag acggcagggg agctccacct 481 ctctaaagtc catggaaggc atggggaagg tgaaagccac ccccatgaca cctgaacaag 541 caatgaagca atacatgcaa aaactcacag ccttcgaaca ccatgagatt ttcagctacc 601 ctgaaatata tttcttgggt ctaaatgcta agaagcgcca gggcatgaca ggtgggccca 661 acaatggtgg ctatgatgat gaccagggat catatgtgca ggtgccccac gatcacgtgg 721 cttacaggta tgaggtcctc aaggtcattg ggaaggggag ctttgggcag gtggtcaagg 781 cctacgatca caaagtccac cagcacgtgg ccctaaagat ggtgcggaat gagaagcgct 841 tccaccggca agcagcggag gagatccgaa tcctggaaca cctgcggaag caggacaagg 901 ataacacaat gaatgtcatc catatgctgg agaatttcac cttccgcaac cacatctgca 961 tgacgtttga gctgctgagc atgaacctct atgagctcat caagaagaat aaattccagg 1021 gcttcagtct gcctttggtt cgcaagtttg cccactcgat tctgcagtgc ttggatgctt 1081 tgcacaaaaa cagaataatt cactgtgacc ttaagcccga gaacattttg ttaaagcagc 1141 agggtagaag cggtattaaa gtaattgatt ttggctccag ttgttacgag catcagcgtg 1201 tctacacgta catccagtcg cgtttttacc gggctccaga agtgatcctt ggggccaggt 1261 atggcatgcc cattgatatg tggagcctgg gctgcatttt agcagagctc ctgacgggtt 1321 accccctctt gcctggggaa gatgaagggg accagctggc ctgtatgatt gaactgttgg 1381 gcatgccctc acagaaactg ctggatgcat ccaaacgagc caaaaatttt gtgagctcca 1441 agggttatcc ccgttactgc actgtcacga ctctctcaga tggctctgtg gtcctaaacg 1501 gaggccgttc ccggaggggg aaactgaggg gcccaccgga gagcagagag tgggggaacg 1561 cgctgaaggg gtgtgatgat ccccttttcc ttgacttctt aaaacagtgt ttagagtggg 1621 atcctgcagt gcgcatgacc ccaggccagg cttTGCGGCA CCCCTGGCTG AGGAGGCGGT 1681 TGCCAAAGCC TCCCACCGGG GAGAAAACGT CAGTGAAAAG GATAACTGAG AGCACCGGTG 1741 CTATCACATC TATATCCAAG TTACCTCCAC CTTCTAGCTC AGCTTCCAAA CTGAGGACTA 1801 ATTTGGCGCA GATGACAGAT GCCAATGGGA ATATTCAGCA GAGGACAGTG TTGCCAAAAC 1861 TTGTTAGCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1921 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1981 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACAAAC GGTCTTTGCT TCCTTTTAAC 2041 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt