Construct: ORF TRCN0000470856
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014193.1_s317c1
- Derived from:
- ccsbBroadEn_13390
- DNA Barcode:
- TGTATGATCACCTAGATTGAGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPNS3 (201305)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470856
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | NM_001320449.2 | 99.7% | 99.7% | 351G>A;399C>T;607G>T |
| 2 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | XM_011523726.2 | 96% | 94.5% | (many diffs) |
| 3 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | XM_011523728.2 | 81.8% | 81.8% | (many diffs) |
| 4 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | NM_182538.5 | 75% | 73.6% | (many diffs) |
| 5 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | XM_011523723.1 | 70.7% | 69.3% | (many diffs) |
| 6 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | XM_011523724.1 | 68.5% | 67.2% | (many diffs) |
| 7 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | XM_017024345.2 | 68.2% | 64.3% | (many diffs) |
| 8 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | XM_011523725.1 | 65.1% | 63.7% | (many diffs) |
| 9 | human | 201305 | SPNS3 | sphingolipid transporter 3 ... | XR_934008.1 | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggtttgcttc agactgtatt cttggctctt cttcctgtcc cggggcatcg 121 tgggcactgg ctcggccagc tactccacca tcgcgcccac cgtcctgggc gacctcttcg 181 tgagggacca gcgcacccgc gtgctggctg tcttctacat ctttatcccc gttggaagtg 241 gtctgggcta cgtgctgggg tcggctgtga cgatgctgac tgggaactgg cgctgggccc 301 tccgagtcat gccctgcctg gaggccgtgg ccttgatcct gcttatcctg ctggttccag 361 acccaccccg gggagctgcc gagacacagg gggagggggc cgtgggaggc ttcagaagca 421 gctggtgtga ggacgtcaga tacctgggga aaaactggag ttttgtgtgg tcgaccctcg 481 gagtgaccgc catggccttt gtgactggag ccctggggtt ctgggccccc aagtttctgc 541 tcgaggcacg cgtggttcac gggctgcagc ctccctgctt ccaggagccg tgcagcaacc 601 ccgacagcct gatttttggg gcactgacca tcatgaccgg cgtcattggg gtcatcttgg 661 gggcagaagc ttcgaggagg tacaagaaag tcattccagg agctgagccc ctcatctgcg 721 cctccagcct gcttgccaca gccccctgcc tctacctggc tctcgtcctg gccccgacca 781 ccctgctggc ctcctatgtg ttcctgggcc ttggggagct gcttctgtcc tgcaactggg 841 cagtggttgc cgacatcctg ctgtctgtgg tggtgcccag atgccggggg acggcagagg 901 cacttcagat cacggtgggc cacatcctgg gagacgctgg cagcccctat ctcacaggac 961 ttatctctag tgtcctgcgg gccaggcgcc ctgactccta tcTGCAGCGC TTCCGCAGCC 1021 TGCAGCAGAG CTTCCTGTGC TGCGCCTTTG TCATCGCCCT GGGGGGCGGC TGCTTCCTGC 1081 TGACTGCGCT GTACCTGGAG AGAGACGAGA CCCGGGCCTG GCAGCCTGTC ACAGGGACCC 1141 CAGACAGCAA TGATGTGGAC AGCAACGACC TGGAGAGACA AGGCCTACTT TCGGGCGCTG 1201 GCGCCTCTAC AGAGGAGCCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGTA TGATCACCTA 1381 GATTGAGATT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt