Construct: ORF TRCN0000470908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001128.1_s317c1
- Derived from:
- ccsbBroadEn_13010
- DNA Barcode:
- CCGTATATTTCTATTTATTTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGS1 (96764)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470908
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 96764 | TGS1 | trimethylguanosine synthase 1 | NM_001363184.2 | 18.5% | 18.5% | 1_1857del |
| 2 | human | 96764 | TGS1 | trimethylguanosine synthase 1 | NM_024831.8 | 16.5% | 16.5% | 1_2136del |
| 3 | mouse | 116940 | Tgs1 | trimethylguanosine synthase 1 | XM_006537558.2 | 16.7% | 17.5% | (many diffs) |
| 4 | mouse | 116940 | Tgs1 | trimethylguanosine synthase 1 | NM_054089.4 | 15% | 15.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agtgattgcc attGATATCG ATCCTGTTAA GATTGCCCTT GCTCGCAATA 121 ATGCAGAAGT TTATGGGATA GCAGATAAGA TAGAGTTCAT CTGTGGAGAT TTCTTGCTGC 181 TGGCTTCTTT TTTAAAGGCT GATGTTGTGT TCCTCAGCCC ACCTTGGGGA GGGCCAGACT 241 ATGCCACTGC AGAGACCTTT GACATTAGAA CAATGATGTC TCCTGATGGC TTTGAAATTT 301 TCAGACTTTC TAAGAAGATC ACTAATAATA TTGTTTATTT TCTTCCAAGA AATGCTGATA 361 TTGACCAGGT GGCATCCTTA GCTGGGCCTG GAGGGCAAGT GGAAATAGAA CAGAACTTCC 421 TTAACAACAA ATTGAAGACA ATCACTGCAT ATTTTGGTGA CCTAATTCGA AGACCAGCCT 481 CTGAAACCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCGTAT ATTTCTATTT ATTTATCGAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt