Construct: ORF TRCN0000470919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015483.1_s317c1
- Derived from:
- ccsbBroadEn_12333
- DNA Barcode:
- CCCGTATCAGGGCTCCAAGGAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOLPP1 (57171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57171 | DOLPP1 | dolichyldiphosphatase 1 | NM_001135917.2 | 49.7% | 49.7% | 1_294del |
| 2 | human | 57171 | DOLPP1 | dolichyldiphosphatase 1 | NM_020438.5 | 40.7% | 40.7% | 1_294del;461_589del |
| 3 | human | 57171 | DOLPP1 | dolichyldiphosphatase 1 | NR_072983.2 | 14.8% | 1_230del;522_1955del | |
| 4 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NM_001290508.1 | 62.6% | 67.1% | (many diffs) |
| 5 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | XM_006498207.3 | 62.6% | 67.1% | (many diffs) |
| 6 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | XM_017319147.1 | 54.7% | 58.7% | (many diffs) |
| 7 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NM_001290509.1 | 44.6% | 48.2% | (many diffs) |
| 8 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NM_020329.4 | 36.8% | 39.4% | (many diffs) |
| 9 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NR_110963.1 | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 70
- ORF end:
- 361
- ORF length:
- 291
- Sequence:
-
1 TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG TTTGTACAAA 61 AAAGTTGGCA TGCCCTCCAG CCATTCCCAG TTTATGTGGT TCTTCTCCGT CTATTCCTTC 121 CTTTTCCTGT ATTTAAGAAT GCACCAAACA AACAACGCCA GGTTCCTGGA CTTGCTGTGG 181 AGGCACGTGC TCTCCCTGGG ACTCCTCGCT GTGGCCTTCC TAGTCTCCTA CAGCAGGCCT 241 GTCTCCGAGT TCTTCCTAAT CCGAGACACA AGCCTCATTC CCAACGTACT CTGGTTTGAG 301 TACACGGTAA CCCGGGCAGA AGCCAGGAAC AGACAACGCA AGCTGGGGAC GAAACTGCAG 361 TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC 421 GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG 481 CTTTATATAT CTTGTGGAAA GGACGACCCG TATCAGGGCT CCAAGGAGGA ACGCGTTAAG 541 TCgacaatca acctctggat tacaaaattt gtgaaagatt