Construct: ORF TRCN0000470920
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017651.1_s317c1
- Derived from:
- ccsbBroadEn_02533
- DNA Barcode:
- TATTGGTAGGCCATTATCAATCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOXA1 (10811)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470920
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | NM_006647.2 | 100% | 100% | |
| 2 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | NM_001256067.2 | 98.5% | 98.5% | 1292_1293ins21 |
| 3 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | XM_011518158.3 | 92.5% | 92.5% | 502_503ins108 |
| 4 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | NM_001256068.1 | 88.4% | 88.4% | 501_502ins168 |
| 5 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | XM_011518159.1 | 69.3% | 69.3% | 0_1ins444 |
| 6 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | XM_011518160.1 | 69.3% | 69.3% | 0_1ins444 |
| 7 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | XM_017014220.1 | 69.3% | 69.3% | 0_1ins444 |
| 8 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | XM_024447388.1 | 67.9% | 67.9% | 0_1ins444;848_849ins21 |
| 9 | human | 10811 | NOXA1 | NADPH oxidase activator 1 | XR_001746169.1 | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1515
- ORF length:
- 1449
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctctctgggg gacctggtgc gcgcctggca cctgggcgcg caggctgtgg 121 atcgtgggga ctgggcccgc gccttgcacc tcttctcggg cgtcccggcg ccgcccgcca 181 ggctgtgctt caacgcgggc tgcgtgcacc tgctggccgg ggaccccgag gccgcgctgc 241 gggcatttga ccaagccgtg accaaggaca cctgcatggc ggttggcttc ttccagcgag 301 gagtggccaa cttccagctg gcaaggttcc aggaggctct gtctgacttc tggctggccc 361 tggagcagct gaggggccac gctgccatcg actacacgca gctgggcctg cggttcaagc 421 tgcaagcctg ggaggtgcta cacaatgtgg cgtcggcaca gtgccagctg gggctctgga 481 cagaggcggc cagcagccta agggaggcca tgtccaagtg gccggagggg tccctgaatg 541 gcctggactc agccctggac caagtgcaga gacggggctc actgccgcca cggcaggtcc 601 ccaggggcga ggtcttccgg ccccaccggt ggcacctgaa gcacttggag cccgtggatt 661 tcctgggcaa ggccaaggtg gtggcctctg ccatccccga cgaccagggc tggggcgtcc 721 gccctcagca gccacaggga ccaggagcga accatgatgc caggtcccta atcatggact 781 ccccaagagc tggcacccac cagggccccc tcgatgcaga gacagaggtc ggtgctgacc 841 gctgcacgtc gactgcctac caggagcaga ggccccaggt ggagcaagtt ggcaaacagg 901 ctcctctctc cccagggctg ccggcaatgg gggggcctgg ccccggcccc tgtgaggacc 961 ccgcgggtgc tgggggagca ggtgcagggg gctccgagcc cctggtgact gtcaccgtgc 1021 agtgcgcctt cacagtggcc ctgagggcac gaagaggagc cgacctgtcc agcctgcggg 1081 cactgctggg ccaagccctc cctcaccagg cccagcttgg gcaactcagt tacctagccc 1141 caggtgagga cgggcactgg gTCCCCATCC CCGAGGAGGA GTCGCTGCAG AGGGCCTGGC 1201 AGGACGCAGC TGCCTGCCCC AGGGGGCTGC AGCTGCAGTG CAGGGGAGCC GGGGGTCGGC 1261 CGGTCCTCTA CCAGGTGGTG GCCCAGCACA GCTACTCCGC CCAGGGGCCA GAGGACCTGG 1321 GCTTCCGACA GGGGGACACG GTGGACGTCC TGTGTGAAGA GCCCGATGTC CCCCTTGCAG 1381 TGGACCAGGC ATGGCTGGAG GGCCACTGTG ACGGCCGCAT CGGCATCTTC CCCAAGTGCT 1441 TCGTGGTCCC CGCCGGCCCT CGGATGTCAG GAGCCCCCGG CCGCCTGCCC CGATCCCAGC 1501 AGGGAGATCA GCCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1561 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1621 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATTGGTAGG CCATTATCAA 1681 TCCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt