Construct: ORF TRCN0000470928
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011385.1_s317c1
- Derived from:
- ccsbBroadEn_02738
- DNA Barcode:
- AGATTGCCTCTATGTGAATTCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RRS1 (23212)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470928
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23212 | RRS1 | ribosome biogenesis regulat... | NM_015169.4 | 100% | 100% | |
| 2 | mouse | 59014 | Rrs1 | ribosome biogenesis regulat... | NM_021511.2 | 88.1% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggccagagc gtggaggagc tgctcgcaaa ggcagagcag gacgaggcag 121 agaagttgca acgcatcacg gtgcacaagg agctggagct gcagtttgac ctgggcaacc 181 tgctggcgtc ggaccggaac cccccgaccg ggctgcggtg cgccggaccc acgccggagg 241 ccgagctaca ggccctggcg cgggacaaca cgcaactgct catcaaccag ctgtggcagc 301 tgcccacgga gcgcgtggaa gaggcgatag tggcgcggct gccggagccc accacacgcc 361 tgccgcgaga gaagcctctg ccccgaccgc ggccacttac acgctggcag cagttcgcgc 421 gcctcaaggg catccgtccc aagaagaaga ccaacctggt gtgggacgag gtgagtggcc 481 agtggcggcg gcgctggggc taccagcgcg cccgggacga caccaaagaa tggctgattg 541 aggtgcccgg caatgccgac cccttggagg accagttcgc caagcggatt caggccaaga 601 aggaaagggt ggccaagaac gagctgaacc ggctgcgtaa cctGGCCCGC GCGCACAAGA 661 TGCAGCTGCC CAGCGCGGCC GGCTTGCACC CTACCGGACA CCAGAGTAAG GAGGAGCTGG 721 GCCGCGCCAT GCAAGTGGCC AAGGTCTCCA CCGCCTCTGT GGGGCGCTTT CAGGAGCGCC 781 TCCCCAAGGA GAAGGTGCCC CGGGGCTCCG GCAAGAAAAG GAAGTTTCAA CCCCTTTTCG 841 GGGACTTTGC AGCCGAGAAA AAGAACCAGT TGGAGCTGCT TCGTGTCATG AACAGCAAGA 901 AGCCTCAGCT GGATGTGACT AGGGCCACCA ATAAGCAGAT GAGGGAGGAG GACCAGGAGG 961 AGGCCGCCAA GAGGAGGAAA ATGAGCCAGA AGGGCAAGAG AAAGGGAGGC CGGCAGGGGC 1021 CTGGGGGCAA GAGGAAAGGG GGCCCGCCCA GCCAGGGAGG GAAGAGGAAA GGGGGCTTGG 1081 GAGGCAAGAT GAATTCTGGG CCGCCTGGCT TGGGTGGCAA GAGAAAAGGA GGACAGCGCC 1141 CAGGAGGAAA GAGGAGGAAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1201 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1261 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGAT TGCCTCTATG 1321 TGAATTCAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt