Construct: ORF TRCN0000470938
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001833.1_s317c1
- Derived from:
- ccsbBroadEn_00036
- DNA Barcode:
- CCGGTGGTCCTATCTCACACCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AFP (174)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470938
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 174 | AFP | alpha fetoprotein | NM_001134.3 | 99.9% | 100% | 1641A>G |
| 2 | human | 174 | AFP | alpha fetoprotein | NM_001354717.2 | 74% | 74% | 0_1ins474;1167A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1893
- ORF length:
- 1827
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gtgggtggaa tcaatttttt taattttcct actaaatttt actgaatcca 121 gaacactgca tagaaatgaa tatggaatag cttccatatt ggattcttac caatgtactg 181 cagagataag tttagctgac ctggctacca tattttttgc ccagtttgtt caagaagcca 241 cttacaagga agtaagcaaa atggtgaaag atgcattgac tgcaattgag aaacccactg 301 gagatgaaca gtcttcaggg tgtttagaaa accagctacc tgcctttctg gaagaacttt 361 gccatgagaa agaaattttg gagaagtacg gacattcaga ctgctgcagc caaagtgaag 421 agggaagaca taactgtttt cttgcacaca aaaagcccac tccagcatcg atcccacttt 481 tccaagttcc agaacctgtc acaagctgtg aagcatatga agaagacagg gagacattca 541 tgaacaaatt catttatgag atagcaagaa ggcatccctt cctgtatgca cctacaattc 601 ttctttgggc tgctcgctat gacaaaataa ttccatcttg ctgcaaagct gaaaatgcag 661 ttgaatgctt ccaaacaaag gcagcaacag ttacaaaaga attaagagaa agcagcttgt 721 taaatcaaca tgcatgtgca gtaatgaaaa attttgggac ccgaactttc caagccataa 781 ctgttactaa actgagtcag aagtttacca aagttaattt tactgaaatc cagaaactag 841 tcctggatgt ggcccatgta catgagcact gttgcagagg agatgtgctg gattgtctgc 901 aggatgggga aaaaatcatg tcctacatat gttctcaaca agacactctg tcaaacaaaa 961 taacagaatg ctgcaaactg accacgctgg aacgtggtca atgtataatt catgcagaaa 1021 atgatgaaaa acctgaaggt ctatctccaa atctaaacag gtttttagga gatagagatt 1081 ttaaccaatt ttcttcaggg gaaaaaaata tcttcttggc aagttttgtt catgaatatt 1141 caagaagaca tcctcagctt gctgtctcag taattctaag agttgctaaa ggataccagg 1201 agttattgga gaagtgtttc cagactgaaa accctcttga atgccaagat aaaggagaag 1261 aagaattaca gaaatacatc caggagagcc aagcattggc aaagcgaagc tgcggcctct 1321 tccagaaact aggagaatat tacttacaaa atgcgtttct cgttgcttac acaaagaaag 1381 ccccccagct gacctcgtcg gagctgatgg ccatcaccag aaaaatggCA GCCACAGCAG 1441 CCACTTGTTG CCAACTCAGT GAGGACAAAC TATTGGCCTG TGGCGAGGGA GCGGCTGACA 1501 TTATTATCGG ACACTTATGT ATCAGACATG AAATGACTCC AGTAAACCCT GGTGTTGGCC 1561 AGTGCTGCAC TTCTTCATAT GCCAACAGGA GGCCATGCTT CAGCAGCTTG GTGGTGGATG 1621 AAACATATGT CCCTCCTGCA TTCTCTGATG ACAAGTTCAT TTTCCATAAG GATCTGTGCC 1681 AAGCTCAGGG TGTAGCGCTG CAAACGATGA AGCAAGAGTT TCTCATTAAC CTTGTGAAGC 1741 AAAAGCCACA AATAACAGAG GAACAACTTG AGGCTGTCAT TGCAGATTTC TCAGGCCTGT 1801 TGGAGAAATG CTGCCAAGGC CAGGAACAGG AAGTCTGCTT TGCTGAAGAG GGACAAAAAC 1861 TGATTTCAAA AACTCGTGCT GCTTTGGGAG TTTACCCAAC TTTCTTGTAC AAAGTGGTTG 1921 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1981 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 2041 GGTGGTCCTA TCTCACACCT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2101 ttgtgaaaga tt