Construct: ORF TRCN0000470938

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001833.1_s317c1
Derived from:
ccsbBroadEn_00036
DNA Barcode:
CCGGTGGTCCTATCTCACACCTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AFP (174)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470938
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 174 AFP alpha fetoprotein NM_001134.3 99.9% 100% 1641A>G
2 human 174 AFP alpha fetoprotein NM_001354717.2 74% 74% 0_1ins474;1167A>G
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1893
ORF length:
1827
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gtgggtggaa tcaatttttt taattttcct actaaatttt actgaatcca
121gaacactgca tagaaatgaa tatggaatag cttccatatt ggattcttac caatgtactg
181cagagataag tttagctgac ctggctacca tattttttgc ccagtttgtt caagaagcca
241cttacaagga agtaagcaaa atggtgaaag atgcattgac tgcaattgag aaacccactg
301gagatgaaca gtcttcaggg tgtttagaaa accagctacc tgcctttctg gaagaacttt
361gccatgagaa agaaattttg gagaagtacg gacattcaga ctgctgcagc caaagtgaag
421agggaagaca taactgtttt cttgcacaca aaaagcccac tccagcatcg atcccacttt
481tccaagttcc agaacctgtc acaagctgtg aagcatatga agaagacagg gagacattca
541tgaacaaatt catttatgag atagcaagaa ggcatccctt cctgtatgca cctacaattc
601ttctttgggc tgctcgctat gacaaaataa ttccatcttg ctgcaaagct gaaaatgcag
661ttgaatgctt ccaaacaaag gcagcaacag ttacaaaaga attaagagaa agcagcttgt
721taaatcaaca tgcatgtgca gtaatgaaaa attttgggac ccgaactttc caagccataa
781ctgttactaa actgagtcag aagtttacca aagttaattt tactgaaatc cagaaactag
841tcctggatgt ggcccatgta catgagcact gttgcagagg agatgtgctg gattgtctgc
901aggatgggga aaaaatcatg tcctacatat gttctcaaca agacactctg tcaaacaaaa
961taacagaatg ctgcaaactg accacgctgg aacgtggtca atgtataatt catgcagaaa
1021atgatgaaaa acctgaaggt ctatctccaa atctaaacag gtttttagga gatagagatt
1081ttaaccaatt ttcttcaggg gaaaaaaata tcttcttggc aagttttgtt catgaatatt
1141caagaagaca tcctcagctt gctgtctcag taattctaag agttgctaaa ggataccagg
1201agttattgga gaagtgtttc cagactgaaa accctcttga atgccaagat aaaggagaag
1261aagaattaca gaaatacatc caggagagcc aagcattggc aaagcgaagc tgcggcctct
1321tccagaaact aggagaatat tacttacaaa atgcgtttct cgttgcttac acaaagaaag
1381ccccccagct gacctcgtcg gagctgatgg ccatcaccag aaaaatggCA GCCACAGCAG
1441CCACTTGTTG CCAACTCAGT GAGGACAAAC TATTGGCCTG TGGCGAGGGA GCGGCTGACA
1501TTATTATCGG ACACTTATGT ATCAGACATG AAATGACTCC AGTAAACCCT GGTGTTGGCC
1561AGTGCTGCAC TTCTTCATAT GCCAACAGGA GGCCATGCTT CAGCAGCTTG GTGGTGGATG
1621AAACATATGT CCCTCCTGCA TTCTCTGATG ACAAGTTCAT TTTCCATAAG GATCTGTGCC
1681AAGCTCAGGG TGTAGCGCTG CAAACGATGA AGCAAGAGTT TCTCATTAAC CTTGTGAAGC
1741AAAAGCCACA AATAACAGAG GAACAACTTG AGGCTGTCAT TGCAGATTTC TCAGGCCTGT
1801TGGAGAAATG CTGCCAAGGC CAGGAACAGG AAGTCTGCTT TGCTGAAGAG GGACAAAAAC
1861TGATTTCAAA AACTCGTGCT GCTTTGGGAG TTTACCCAAC TTTCTTGTAC AAAGTGGTTG
1921ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
1981GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC
2041GGTGGTCCTA TCTCACACCT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat
2101ttgtgaaaga tt

Download FASTA (ORF) (Full)