Construct: ORF TRCN0000470957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007520.1_s317c1
- Derived from:
- ccsbBroadEn_04315
- DNA Barcode:
- GCTCAACACCGTCGGGGTGTGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- REG4 (83998)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470957
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83998 | REG4 | regenerating family member 4 | NM_001159352.2 | 100% | 100% | |
| 2 | human | 83998 | REG4 | regenerating family member 4 | NM_032044.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttccagaagc atgcggctgc TCCTATTGCT GAGCTGCCTG GCCAAAACAG 121 GAGTCCTGGG TGATATCATC ATGAGACCCA GCTGTGCTCC TGGATGGTTT TACCACAAGT 181 CCAATTGCTA TGGTTACTTC AGGAAGCTGA GGAACTGGTC TGATGCCGAG CTCGAGTGTC 241 AGTCTTACGG AAACGGAGCC CACCTGGCAT CTATCCTGAG TTTAAAGGAA GCCAGCACCA 301 TAGCAGAGTA CATAAGTGGC TATCAGAGAA GCCAGCCGAT ATGGATTGGC CTGCACGACC 361 CACAGAAGAG GCAGCAGTGG CAGTGGATTG ATGGGGCCAT GTATCTGTAC AGATCCTGGT 421 CTGGCAAGTC CATGGGTGGG AACAAGCACT GTGCTGAGAT GAGCTCCAAT AACAACTTTT 481 TAACTTGGAG CAGCAACGAA TGCAACAAGC GCCAACACTT CCTGTGCAAG TACCGACCAT 541 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGAGCTCA ACACCGTCGG GGTGTGCTGA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt