Transcript: Human NM_001159352.2

Homo sapiens regenerating family member 4 (REG4), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
REG4 (83998)
Length:
1398
CDS:
321..797

Additional Resources:

NCBI RefSeq record:
NM_001159352.2
NBCI Gene record:
REG4 (83998)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001159352.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000145209 GATGAGCTCCAATAACAACTT pLKO.1 713 CDS 100% 4.950 3.960 N REG4 n/a
2 TRCN0000122111 CCTGGCTAAATCTGCTCATTA pLKO.1 856 3UTR 100% 13.200 9.240 N REG4 n/a
3 TRCN0000141053 CAACACTTCCTGTGCAAGTAC pLKO.1 768 CDS 100% 4.950 3.465 N REG4 n/a
4 TRCN0000122256 CCTAGCAAACTAAGAGTGATA pLKO.1 891 3UTR 100% 4.950 3.465 N REG4 n/a
5 TRCN0000142464 GAACAAGCACTGTGCTGAGAT pLKO.1 695 CDS 100% 4.950 3.465 N REG4 n/a
6 TRCN0000142058 GAGCAGCAACGAATGCAACAA pLKO.1 743 CDS 100% 4.950 3.465 N REG4 n/a
7 TRCN0000141471 CCTATGAAACAGCTGGGTCTT pLKO.1 1082 3UTR 100% 4.050 2.835 N REG4 n/a
8 TRCN0000122006 GCAGAGTACATAAGTGGCTAT pLKO.1 558 CDS 100% 4.050 2.835 N REG4 n/a
9 TRCN0000145009 GCAAGAATCAAGATTCTGCTA pLKO.1 799 3UTR 100% 0.264 0.185 N REG4 n/a
10 TRCN0000189498 CCAACACTTCCTGTGCAAGTA pLKO.1 767 CDS 100% 4.950 2.970 N Reg4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001159352.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04315 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04315 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000470957 GCTCAACACCGTCGGGGTGTGCTG pLX_317 93.1% 100% 100% V5 n/a
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