Construct: ORF TRCN0000470970
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007030.1_s317c1
- Derived from:
- ccsbBroadEn_03369
- DNA Barcode:
- ACTTTCTCATACATTGAAAAGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYB5R1 (51706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470970
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51706 | CYB5R1 | cytochrome b5 reductase 1 | NM_016243.3 | 100% | 100% | |
| 2 | mouse | 72017 | Cyb5r1 | cytochrome b5 reductase 1 | NM_028057.3 | 89.5% | 91.8% | (many diffs) |
| 3 | mouse | 72017 | Cyb5r1 | cytochrome b5 reductase 1 | NM_001355133.1 | 80.2% | 81.3% | (many diffs) |
| 4 | mouse | 72017 | Cyb5r1 | cytochrome b5 reductase 1 | NR_149307.1 | 33.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gatccagacg agccccgtcc tgctggcctc cctgggggtg gggctggtca 121 ctctgctcgg cctggctgtg ggctcctact tggttcggag gtcccgccgg cctcaggtca 181 ctctcctgga ccccaatgaa aagtacctgc tacgactgct agacaagacg actgtgagcc 241 acaacaccaa gaggttccgc tttgccctgc ccaccgccca ccacactctg gggctgcctg 301 tgggcaaaca tatctacctc tccacccgaa ttgatggcag cctggtcatc aggccataca 361 ctcctgtcac cagtgatgag gatcaaggct atgtggatct tgtcatcaag gtctacctga 421 agggtgtgca ccccaaattt cctgagggag ggaagatgtc tcagtacctg gatagcctga 481 aggttgggga tgtggtggag tttcgggggc caagcgggtt gctcacttac actggaaaag 541 ggcattttaa cattcagccc aacaagaaat ctccaccaga accccgagtg gcgaagaaac 601 tgggaatgat tgccggcggg acaggaatca ccccaatgct acagctgatc cgggccatcc 661 TGAAAGTCCC TGAAGATCCA ACCCAGTGCT TTCTGCTTTT TGCCAACCAG ACAGAAAAGG 721 ATATCATCTT GCGGGAGGAC TTAGAGGAAC TGCAGGCCCG CTATCCCAAT CGCTTTAAGC 781 TCTGGTTCAC TCTGGATCAT CCCCCAAAAG ATTGGGCCTA CAGCAAGGGC TTTGTGACTG 841 CCGACATGAT CCGGGAACAC CTGCCCGCTC CAGGGGATGA TGTGCTGGTA CTGCTTTGTG 901 GGCCACCCCC AATGGTGCAG CTGGCCTGCC ATCCCAACTT GGACAAACTG GGCTACTCAC 961 AAAAGATGCG ATTCACCTAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTT TCTCATACAT 1141 TGAAAAGATT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt