Construct: ORF TRCN0000471003
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006088.1_s317c1
- Derived from:
- ccsbBroadEn_00753
- DNA Barcode:
- CTCACCACCTATTTCTTGGTGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN1 (3150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471003
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3150 | HMGN1 | high mobility group nucleos... | NM_004965.7 | 100% | 100% | |
| 2 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_011529543.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 3 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_011529544.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 4 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_011529549.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 5 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028333.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 6 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028334.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 7 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028335.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 8 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_017028336.2 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 9 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452067.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 10 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452068.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 11 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452069.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 12 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452070.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 13 | human | 3150 | HMGN1 | high mobility group nucleos... | XM_024452071.1 | 28.2% | 4.4% | 0_1ins123;2_15del;192_504del |
| 14 | human | 10230 | NBR2 | neighbor of BRCA1 lncRNA 2 | NR_003108.2 | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 366
- ORF length:
- 300
- Sequence:
-
1 tcttccattT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCC CAAGAGGAAG GTCAGCTCCG CCGAAGGCGC CGCCAAGGAA GAGCCCAAGA 121 GGAGATCGGC GCGGTTGTCA GCTAAACCTC CTGCAAAAGT GGAAGCGAAG CCGAAAAAGG 181 CAGCAGCGAA GGATAAATCT TCAGACAAAA AAGTGCAAAC AAAAGGGAAA AGGGGAGCAA 241 AGGGAAAACA GGCCGAAGTG GCTAACCAAG AAACTAAAGA AGACTTACCT GCGGAAAACG 301 GGGAAACGAA GACTGAGGAG AGTCCAGCCT CTGATGAAGC AGGAGAGAAA GAAGCCAAGT 361 CTGATTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTCACCACC TATTTCTTGG TGTCCACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt