Construct: ORF TRCN0000471006

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004444.1_s317c1
Derived from:
ccsbBroadEn_03722
DNA Barcode:
TAATGATATGGCATCATTCTATTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CTPS2 (56474)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471006
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56474 CTPS2 CTP synthase 2 NM_001144002.1 100% 100%
2 human 56474 CTPS2 CTP synthase 2 NM_019857.5 100% 100%
3 human 56474 CTPS2 CTP synthase 2 NM_175859.3 100% 100%
4 human 56474 CTPS2 CTP synthase 2 XM_005274562.3 100% 100%
5 human 56474 CTPS2 CTP synthase 2 XM_006724503.3 100% 100%
6 human 56474 CTPS2 CTP synthase 2 XM_024452408.1 100% 100%
7 human 56474 CTPS2 CTP synthase 2 XM_024452409.1 100% 100%
8 human 56474 CTPS2 CTP synthase 2 XM_011545545.2 93.3% 93.3% 1_126del
9 human 56474 CTPS2 CTP synthase 2 XR_002958784.1 42.5% 1_374del;1670_1853del;2317_4131del
10 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... NM_001168568.1 87.6% 90.4% (many diffs)
11 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... NM_001168569.1 87.6% 90.4% (many diffs)
12 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... NM_018737.5 87.6% 90.4% (many diffs)
13 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... XM_006528912.2 87.6% 90.4% (many diffs)
14 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... XM_006528913.2 87.6% 90.4% (many diffs)
15 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... XM_006528911.2 80.9% 83.4% (many diffs)
16 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... NM_001168571.1 80.6% 83.6% (many diffs)
17 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... XM_006528915.1 68.1% 69.2% (many diffs)
18 mouse 55936 Ctps2 cytidine 5'-triphosphate sy... NR_033143.1 28.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1824
ORF length:
1758
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gtacatcctg gtcacgggtg gggtcatctc aggcattggt aaagggatca
121ttgccagcag cattggaacg attctaaaat catgtggact ccgagttact gccataaaaa
181tcgaccccta tattaacatc gatgctggca ctttttcacc ttatgaacac ggtgaagtct
241tcgtcttaaa tgatggtgga gaagttgatt tagaccttgg aaattatgaa agatttttgg
301atattaatct ttataaagac aacaatatca ccacggggaa gatatatcag catgtgatca
361ataaagagag gcgtggtgat tacctgggga aaacagtgca agttgtccct cacattactg
421atgctgtcca ggagtgggtt atgaatcaag ccaaggtgcc ggtggatggt aataaggaag
481agccccaaat atgcgttatt gagctgggag gcaccattgg agacatcgaa ggaatgccgt
541ttgtggaggc gtttagacaa ttccagttta aggcgaaaag agagaatttc tgtaatatcc
601acgttagcct tgtcccacag ctcagtgcta ccggagaaca aaaaaccaaa cccacccaaa
661acagcgtccg cgcactgagg ggtttaggcc tgtctccaga tctgattgtc tgccgaagtt
721caacgcccat tgagatggcc gtgaaggaga agatttctat gttttgtcac gtgaaccctg
781aacaggtcat atgtatccat gatgtttctt ccacataccg agttcctgtg cttttagagg
841aacaaagcat tgtgaaatat tttaaggaga gattgcacct gcccatcggt gattctgcaa
901gtaatttgct ttttaagtgg agaaatatgg ctgacaggta tgaaaggtta cagaaaatat
961gctccatagc cctggttggc aaatacacca agctcagaga ctgctacgcc tctgtgttca
1021aagccctgga acactcagcc ctggccatca accacaagtt gaatctgatg tacatagact
1081ccattgatct ggagaagatc actgaaaccg aggaccctgt gaaatttcat gaagcttggc
1141agaagctatg caaagctgat ggtattcttg tgcctggagg ctttggaatc agaggaacat
1201tgggaaaact ccaggcgatt tcttgggcaa ggacaaagaa gattcctttt ctgggagttt
1261gtcttgggat gcaactagca gtgatagagt ttgcaagaaa ctgccttaac ttgaaagatg
1321ctgattccac agagtttagg ccaaatgccc cagttcctct ggtgattgat atgcccgagc
1381acaacccTGG CAATTTGGGA GGAACAATGA GACTGGGAAT AAGAAGAACT GTTTTCAAAA
1441CTGAAAATTC AATATTAAGG AAACTTTATG GTGATGTTCC TTTTATAGAA GAAAGACACA
1501GACATCGGTT CGAGGTAAAC CCTAACCTGA TCAAACAATT TGAGCAGAAT GACTTAAGTT
1561TTGTAGGTCA GGATGTTGAT GGAGACAGGA TGGAAATCAT TGAACTGGCA AATCATCCTT
1621ATTTTGTTGG TGTCCAGTTC CATCCTGAGT TTTCTTCTAG GCCGATGAAG CCTTCCCCTC
1681CGTATCTGGG GCTGTTACTT GCAGCAACTG GGAACCTGAA TGCCTACTTG CAACAGGGTT
1741GCAAACTGTC TTCCAGTGAT AGATACAGTG ATGCCAGTGA TGACAGCTTT TCAGAGCCAA
1801GGATAGCTGA GTTGGAAATA AGCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1861AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1921TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATGATATGG
1981CATCATTCTA TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2041att

Download FASTA (ORF) (Full)