Construct: ORF TRCN0000471037
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016400.1_s317c1
- Derived from:
- ccsbBroadEn_04831
- DNA Barcode:
- AGGCGAGGTAAGTCATCTGCTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL8A (127829)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471037
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127829 | ARL8A | ADP ribosylation factor lik... | NM_138795.4 | 100% | 100% | |
| 2 | human | 127829 | ARL8A | ADP ribosylation factor lik... | NM_001256129.1 | 79% | 68.6% | 372_373ins68;441_442ins49 |
| 3 | human | 55207 | ARL8B | ADP ribosylation factor lik... | NM_018184.3 | 77.2% | 91.9% | (many diffs) |
| 4 | mouse | 68724 | Arl8a | ADP-ribosylation factor-lik... | NM_026823.2 | 92.4% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 624
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cgctttgttc aacaagctgc tggactggtt caaggcccta ttctggaagg 121 aggagatgga gctcacgctg gtcgggcttc agtactcggg caagaccacc ttcgtcaacg 181 tgatcgcgtc aggacagttc aacgaggaca tgaTCCCCAC CGTGGGTTTC AACATGCGCA 241 AAATCACCAA AGGGAATGTG ACTATCAAGC TCTGGGACAT TGGGGGACAG CCGCGTTTCC 301 GCAGCATGTG GGAGCGCTAC TGCCGAGGAG TGAGCGCCAT CGTGTACATG GTGGATGCTG 361 CTGACCAGGA GAAGATTGAG GCCTCTAAGA ACGAGCTCCA CAACCTACTG GACAAACCTC 421 AGCTGCAGGG CATCCCGGTC TTAGTCCTGG GTAACAAGCG AGACCTTCCG GGAGCATTGG 481 ATGAGAAGGA GCTGATTGAG AAAATGAATC TGTCTGCCAT CCAGGACCGA GAGATCTGCT 541 GCTACTCCAT CTCTTGCAAA GAAAAGGACA ACATTGACAT CACCCTACAG TGGCTTATTC 601 AACACTCGAA GTCACGGAGA AGCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 661 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 721 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GGCGAGGTAA 781 GTCATCTGCT ATGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 841 att