Construct: ORF TRCN0000471039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007025.1_s317c1
- Derived from:
- ccsbBroadEn_14242
- DNA Barcode:
- TTTATATAAATTCTCAGTTGCATG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCCC2 (64087)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | XM_017009688.1 | 83.7% | 79.2% | (many diffs) |
| 2 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | XM_011543529.2 | 82.6% | 80.7% | (many diffs) |
| 3 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | NM_001363147.1 | 61.8% | 60.9% | (many diffs) |
| 4 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | NM_022132.5 | 57.7% | 56.8% | (many diffs) |
| 5 | human | 64087 | MCCC2 | methylcrotonoyl-CoA carboxy... | XR_001742172.1 | 26.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1053
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggccgtcctg aggttagccc tgcggccgtg tgcccgcgcc tctcccgccg 121 ggccgcgcgc ctatcacggg gactcggtgg cctcgctggg cacccagccg gacttgggct 181 ctgccctcta ccaggagaac tacaagcaga tgaaagcact agtaaatcag ctccatgaac 241 gagtggagca tataaaacta ggaggtggtg agaaagcccg agcacttcac atatcaagag 301 gaaaactatt gcccagagaa agaattgaca atctcataga cccagggtct ccatttctgg 361 aattatccca gtttgcaggt taccagttat atgacaatga ggaggtgcca ggaggtggca 421 ttattacagg cattggaaga gtatcaggag tagaatgcat gattattgcc aatgatgcca 481 ccgtcaaagg aggtgcctac tacccagtga ctgtgaaaaa acaattacgg gcccaagaaa 541 ttgccatgca aaacaggctc ccctgcatct acttagttga ttcgggagga gcatacttac 601 ctcgacaagc agatgtgttt ccagatcgag accactttgg ccgtacattc tataatcagg 661 caattatgtc ttctaaaaat attgcacagg ttaaagcggc aactgggGAA GAAGTATCTG 721 CTGAGGATCT TGGAGGTGCT GATCTTCATT GCAGAAAGTC TGGAGTAAGT GACCACTGGG 781 CTTTGGATGA TCATCATGCC CTTCACTTAA CTAGGAAGGT TGTGAGGAAT CTAAATTATC 841 AGAAGAAATT GGATGTCACC ATTGAACCTT CTGAAGAGCC TTTATTTCCT GCTGATGAAT 901 TGTATGGAAT AGTTGGTGCT AACCTTAAGA GGAGCTTTGA TGTCCGAGAG GTCATTGCTA 961 GAATCGTGGA TGGAAGCAGA TTCACTGAGT TCAAAGCCTT TTATGGAGAC ACATTAGTTA 1021 CAGGTATAAA GCCCATGGAA AAGCACAAGA CAACCCAACT TTCTTGTACA AAGTGGTTGA 1081 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1141 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT 1201 ATATAAATTC TCAGTTGCAT GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1261 tgtgaaagat t