Construct: ORF TRCN0000471066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007694.1_s317c1
- Derived from:
- ccsbBroadEn_03976
- DNA Barcode:
- CCGTCCCAGTATAAATTCTCAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PORCN (64840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64840 | PORCN | porcupine O-acyltransferase | NM_203473.3 | 100% | 100% | |
| 2 | human | 64840 | PORCN | porcupine O-acyltransferase | NM_203474.1 | 98.9% | 98.9% | (many diffs) |
| 3 | human | 64840 | PORCN | porcupine O-acyltransferase | NM_203475.3 | 98.9% | 98.9% | 703_717del |
| 4 | human | 64840 | PORCN | porcupine O-acyltransferase | NM_022825.4 | 98.6% | 98.4% | 686_687insCAAGAAACGCAAAGCCAG |
| 5 | human | 64840 | PORCN | porcupine O-acyltransferase | NM_001282167.2 | 83.1% | 82.8% | 0_1ins213;473_474insCAAGAAACGCAAAGCCAG |
| 6 | human | 64840 | PORCN | porcupine O-acyltransferase | XM_024452425.1 | 65.8% | 60% | (many diffs) |
| 7 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | NM_145908.4 | 93.5% | 97.1% | (many diffs) |
| 8 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | NM_145907.4 | 92.5% | 96% | (many diffs) |
| 9 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | NM_023638.4 | 92.5% | 96% | (many diffs) |
| 10 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | NM_016913.4 | 92.2% | 95.6% | (many diffs) |
| 11 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | XM_006527644.2 | 84.9% | 88.2% | (many diffs) |
| 12 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | XM_006527645.2 | 62.8% | 65.1% | (many diffs) |
| 13 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | XR_878030.2 | 46.7% | (many diffs) | |
| 14 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | NM_001308474.1 | 45.9% | 47.5% | (many diffs) |
| 15 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | XM_011247481.1 | 45.8% | 47.3% | (many diffs) |
| 16 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | NR_131893.1 | 43.5% | (many diffs) | |
| 17 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | XR_001782756.1 | 40.6% | (many diffs) | |
| 18 | mouse | 53627 | Porcn | porcupine homolog (Drosophila) | XR_001782757.1 | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1434
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacctttagc cgccaggaat ttttccagca gctactgcaa ggctgtctcc 121 tgcctactgc ccagcagggc cttgaccaga tctggctgct ccttgccatc tgcctcgcct 181 gccgcctcct ctggaggctc gggttgccat cctacctgaa gcatgcaagc accgtggcag 241 gcgggttctt cagcctctac cacttcttcc agctgcacat ggtttgggtc gtgctgctca 301 gcctcctgtg ctacctcgtg ctgttcctct gccgacattc ctcccatcga ggcgtcttcc 361 tatccgtcac catcctcatc tacctactca tgggtgagat gcacatggta gacaccgtga 421 catggcacaa gatgcgaggg gcacagatga ttgtggccat gaaggcagtg tctctgggct 481 tcgacctgga ccggggcgag gtgggtacgg tgccctcgcc agtggagttc atgggctacc 541 tctacttcgt gggcaccatc gtcttcgggc cctggatatc cttccacagc tacctacaag 601 ctgtccaagg ccgcccactg agctgccggt ggctgcagaa ggtggcccgg agcctggcac 661 tggccctgct gtgccttgtg ctgtccactt gcgtgggccc ctacctcttc ccgtacttca 721 tccccctcaa cggtgaccgc ctccttcgca acaagaaacg caaagccagg tggctgcgag 781 cctacgagag tgctgtctcc ttccacttca gcaactattt tgtgggcttt ctttccgagg 841 ccacggccac gttggcgggg gctggcttta ccgaggagaa ggatcacctg gaatgggacc 901 tgacggtgtc caagccactg aatgtggagc tgcctcggtc aatggtggaa gttgtcacaa 961 gctggaacct gcccatgtct tattggctaa ataactatgt tttcaagaat gctctccgcc 1021 tggggacctt cTCGGCTGTG CTGGTCACCT ATGCAGCCAG CGCCCTCCTA CATGGCTTCA 1081 GTTTCCACCT GGCTGCGGTC CTGCTGTCCC TGGCTTTTAT CACTTACGTG GAGCATGTCC 1141 TCCGGAAGCG CCTGGCTCGG ATCCTCAGTG CCTGTGTCTT GTCAAAGCGG TGCCCGCCAG 1201 ACTGTTCGCA CCAGCATCGC TTGGGCCTGG GGGTGCGAGC CTTAAACTTG CTCTTTGGAG 1261 CTCTGGCCAT CTTCCACCTG GCCTACCTGG GCTCCCTGTT TGATGTCGAT GTGGATGACA 1321 CCACAGAGGA GCAGGGCTAC GGCATGGCAT ACACTGTCCA CAAGTGGTCA GAGCTCAGCT 1381 GGGCCAGTCA CTGGGTCACT TTTGGATGCT GGATCTTCTA CCGTCTCATA GGCTGCCCAA 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAC CGTCCCAGTA TAAATTCTCA ATGACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att