Construct: ORF TRCN0000471074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001005.1_s317c1
- Derived from:
- ccsbBroadEn_07308
- DNA Barcode:
- AACTCCAAGCCCAGTTCCCACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPM1 (8813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471074
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8813 | DPM1 | dolichyl-phosphate mannosyl... | NM_003859.2 | 99.6% | 99.2% | 15A>G;25A>G;658T>A |
| 2 | human | 8813 | DPM1 | dolichyl-phosphate mannosyl... | NM_001317036.1 | 90.7% | 90.3% | (many diffs) |
| 3 | human | 8813 | DPM1 | dolichyl-phosphate mannosyl... | NM_001317035.1 | 90.2% | 89.8% | (many diffs) |
| 4 | human | 8813 | DPM1 | dolichyl-phosphate mannosyl... | NM_001317034.1 | 87.7% | 87.4% | (many diffs) |
| 5 | human | 8813 | DPM1 | dolichyl-phosphate mannosyl... | XR_002958550.1 | 69.1% | (many diffs) | |
| 6 | human | 8813 | DPM1 | dolichyl-phosphate mannosyl... | NR_133648.1 | 69% | (many diffs) | |
| 7 | human | 8813 | DPM1 | dolichyl-phosphate mannosyl... | XR_002958551.1 | 62% | (many diffs) | |
| 8 | mouse | 13480 | Dpm1 | dolichol-phosphate (beta-D)... | NM_010072.4 | 87.5% | 91.1% | (many diffs) |
| 9 | mouse | 13480 | Dpm1 | dolichol-phosphate (beta-D)... | NM_001310084.1 | 79.8% | 82.6% | (many diffs) |
| 10 | mouse | 105980075 | Gm20716 | predicted gene 20716 | NR_132310.1 | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 846
- ORF length:
- 780
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccttggag gtcagtcgtg gtcctcgcag gtctcggcgg gagctggaag 121 tgcgcagtcc acgacagaac aaatattcgg tgcttttacc tacctacaac gagcgcgaga 181 acctgccgct catcgtgtgg ctgctggtga aaagcttctc cgagagtgga atcaactatg 241 aaattataat catagatgat ggaagcccag atggaacaag ggatgttgct gaacagttgg 301 agaagatcta tgggtcagac agaattcttc taagaccacg agagaaaaag ttGGGACTAG 361 GAACTGCATA TATTCATGGA ATGAAACATG CCACAGGAAA CTACATCATT ATTATGGATG 421 CTGATCTCTC ACACCATCCA AAATTTATTC CTGAATTTAT TAGGAAGCAA AAGGAGGGTA 481 ATTTTGATAT TGTCTCTGGA ACTCGCTACA AAGGAAATGG AGGTGTATAT GGCTGGGATT 541 TGAAAAGAAA AATAATCAGC CGTGGGGCCA ATTTTTTAAC TCAGATCTTG CTGAGACCAG 601 GAGCATCTGA TTTAACAGGA AGTTTCAGAT TATACCGAAA AGAAGTTCTA GAGAAATTAA 661 TAGAAAAATG TGTTTCTAAA GGCTACGTCT TCCAGATGGA GATGATTGTT CGGGCAAGAC 721 AGATGAATTA TACTATTGGC GAGGTTCCAA TATCATTTGT GGATCGTGTT TATGGTGAAT 781 CCAAGTTGGG AGGAAATGAA ATAGTATCTT TCTTGAAAGG ATTATTGACT CTTTTTGCTA 841 CTACATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 901 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 961 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACTCCAAG CCCAGTTCCC ACCCCACGCG 1021 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt