Transcript: Human XR_002958550.1

PREDICTED: Homo sapiens dolichyl-phosphate mannosyltransferase subunit 1, catalytic (DPM1), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DPM1 (8813)
Length:
1123
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002958550.1
NBCI Gene record:
DPM1 (8813)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002958550.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218642 GTCCACGACAGAACAAATATT pLKO_005 100 3UTR 100% 15.000 21.000 N DPM1 n/a
2 TRCN0000229294 ATCATTTGTGGATCGTGTTTA pLKO_005 750 3UTR 100% 13.200 18.480 N DPM1 n/a
3 TRCN0000229293 TCGGGCAAGACAGTTGAATTA pLKO_005 708 3UTR 100% 13.200 18.480 N DPM1 n/a
4 TRCN0000229292 GGACTAGGAACTGCATATATT pLKO_005 327 3UTR 100% 15.000 10.500 N DPM1 n/a
5 TRCN0000036184 CCACAGGAAACTACATCATTA pLKO.1 364 3UTR 100% 13.200 9.240 N DPM1 n/a
6 TRCN0000036186 GCAGTCCACGACAGAACAAAT pLKO.1 97 3UTR 100% 13.200 9.240 N DPM1 n/a
7 TRCN0000229295 TTACGTTCATTTCAGGTTAAA pLKO_005 868 3UTR 100% 13.200 9.240 N DPM1 n/a
8 TRCN0000036188 TGAATCCAAGTTGGGAGGAAA pLKO.1 774 3UTR 100% 4.950 3.465 N DPM1 n/a
9 TRCN0000036185 GAGGTGTATATGGCTGGGATT pLKO.1 493 3UTR 100% 4.050 2.835 N DPM1 n/a
10 TRCN0000036187 GTTCCAATATCATTTGTGGAT pLKO.1 742 3UTR 100% 2.640 1.848 N DPM1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002958550.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07309 pDONR223 100% 69.3% None (many diffs) n/a
2 ccsbBroad304_07309 pLX_304 0% 69.3% V5 (many diffs) n/a
3 TRCN0000467398 TCAGGTGCATTTAGCAAGACACTC pLX_317 59.6% 69.3% V5 (many diffs) n/a
4 ccsbBroadEn_07308 pDONR223 100% 69.1% None (many diffs) n/a
5 ccsbBroad304_07308 pLX_304 0% 69.1% V5 (many diffs) n/a
6 TRCN0000471074 AACTCCAAGCCCAGTTCCCACCCC pLX_317 63% 69.1% V5 (many diffs) n/a
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