Construct: ORF TRCN0000471079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017013.1_s317c1
- Derived from:
- ccsbBroadEn_02764
- DNA Barcode:
- GATCCCCTTTATACCTCCTTTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMED3 (23423)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471079
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23423 | TMED3 | transmembrane p24 trafficki... | NM_007364.4 | 99.8% | 100% | 516A>G |
| 2 | human | 23423 | TMED3 | transmembrane p24 trafficki... | NM_001330376.1 | 73.3% | 61.3% | (many diffs) |
| 3 | human | 23423 | TMED3 | transmembrane p24 trafficki... | NM_001301203.2 | 68.8% | 65.8% | (many diffs) |
| 4 | human | 23423 | TMED3 | transmembrane p24 trafficki... | NM_001363735.1 | 64.8% | 64.2% | (many diffs) |
| 5 | human | 23423 | TMED3 | transmembrane p24 trafficki... | NR_125394.1 | 40.3% | (many diffs) | |
| 6 | mouse | 66111 | Tmed3 | transmembrane p24 trafficki... | NM_025360.2 | 84% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cagcactgtc ccgcgctccg cctccgtgct gcttctgctg ctgctcctgc 121 gccgggccga gcagccctgc ggggccgagc tcaccttcga gctgCCGGAC AACGCCAAGC 181 AGTGCTTCCA CGAGGAGGTG GAGCAGGGCG TGAAGTTCTC CCTGGATTAC CAGGTCATCA 241 CTGGAGGCCA CTACGATGTT GACTGCTATG TAGAGGACCC CCAGGGGAAC ACCATCTACA 301 GAGAAACGAA GAAGCAGTAC GACAGCTTCA CGTACCGGGC TGAAGTCAAG GGCGTTTATC 361 AGTTTTGCTT CAGTAATGAG TTTTCCACCT TCTCTCACAA GACCGTCTAC TTTGACTTTC 421 AAGTGGGCGA TGAGCCTCCC ATTCTCCCAG ACATGGGGAA CAGGGTCACA GCTCTCACCC 481 AGATGGAGTC CGCCTGCGTG ACCATCCATG AGGCTCTGAA AACGGTGATT GACTCCCAGA 541 CGCATTACCG GCTGCGGGAG GCCCAGGACC GGGCCCGAGC GGAAGACCTT AATAGCCGAG 601 TCTCTTACTG GTCTGTTGGC GAGACGATTG CCCTGTTCGT GGTCAGCTTC AGTCAGGTGC 661 TACTGTTGAA AAGCTTCTTC ACAGAAAAAC GACCCATCAG CAGGGCAGTC CACTCCTACC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAGATCCCCT TTATACCTCC TTTTGAACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt