Construct: ORF TRCN0000471084
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002735.1_s317c1
- Derived from:
- ccsbBroadEn_12771
- DNA Barcode:
- TGCTCTGTATAATTGACACACCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDHD2 (84064)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471084
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | NM_001318765.2 | 100% | 100% | |
| 2 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_006722557.2 | 100% | 100% | |
| 3 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_017026040.1 | 83% | 81.6% | (many diffs) |
| 4 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | NM_032124.5 | 65.2% | 65.2% | 1_270del |
| 5 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_011526227.2 | 65.2% | 65.2% | 1_270del |
| 6 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_011526228.3 | 65.2% | 65.2% | 1_270del |
| 7 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_017026039.1 | 65.2% | 65.2% | 1_270del |
| 8 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_005258371.4 | 54.4% | 53.2% | (many diffs) |
| 9 | human | 84064 | HDHD2 | haloacid dehalogenase like ... | XM_024451272.1 | 54.4% | 53.2% | (many diffs) |
| 10 | mouse | 76987 | Hdhd2 | haloacid dehalogenase-like ... | NM_001039201.1 | 56.5% | 59% | (many diffs) |
| 11 | mouse | 76987 | Hdhd2 | haloacid dehalogenase-like ... | NM_029826.2 | 56.5% | 59% | (many diffs) |
| 12 | mouse | 76987 | Hdhd2 | haloacid dehalogenase-like ... | XM_006526442.3 | 56.5% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctagttgat gatcgggcac tacctgattt caaaggaata caaacaagtg 121 aTCCTAATGC TGTGGTCATG GGATTGGCAC CAGAACATTT TCATTATCAA ATTCTGAATC 181 AAGCATTCCG GTTACTCCTG GATGGAGCAC CTCTGATAGC AATCCACAAA GCCAGGTATT 241 ACAAGAGGAA AGATGGCTTA GCCCTGGGGC CTGGACCATT TGTGACTGCT TTAGAGTATG 301 CCACAGATAC CAAAGCCACA GTCGTGGGGA AACCAGAGAA GACGTTCTTT TTGGAAGCAT 361 TGCGGGGCAC TGGCTGTGAA CCTGAGGAGG CTGTCATGAT AGGAGATGAT TGCAGGGATG 421 ATGTTGGTGG GGCTCAAGAT GTCGGCATGC TGGGCATCTT AGTAAAGACT GGGAAATATC 481 GAGCATCAGA TGAAGAAAAA ATTAATCCAC CTCCTTACTT AACTTGTGAG AGTTTCCCTC 541 ATGCTGTGGA CCACATTCTG CAGCACCTAT TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 721 CTCTGTATAA TTGACACACC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt