Construct: ORF TRCN0000471100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006354.1_s317c1
- Derived from:
- ccsbBroadEn_02774
- DNA Barcode:
- ATTGCTCTTCACTATCCAATAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETHE1 (23474)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471100
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23474 | ETHE1 | ETHE1 persulfide dioxygenase | NM_014297.5 | 100% | 100% | |
| 2 | human | 23474 | ETHE1 | ETHE1 persulfide dioxygenase | NM_001320867.2 | 95.6% | 95.6% | 225_226ins33 |
| 3 | human | 23474 | ETHE1 | ETHE1 persulfide dioxygenase | XM_005258687.4 | 89.3% | 89.3% | 0_1ins81 |
| 4 | human | 23474 | ETHE1 | ETHE1 persulfide dioxygenase | NM_001320869.2 | 61.4% | 61.4% | 81_82ins294 |
| 5 | human | 23474 | ETHE1 | ETHE1 persulfide dioxygenase | NM_001320868.2 | 51.3% | 50.7% | (many diffs) |
| 6 | mouse | 66071 | Ethe1 | ethylmalonic encephalopathy 1 | NM_001364014.1 | 84.4% | 90.1% | (many diffs) |
| 7 | mouse | 66071 | Ethe1 | ethylmalonic encephalopathy 1 | NM_023154.4 | 84.4% | 90.1% | (many diffs) |
| 8 | mouse | 66071 | Ethe1 | ethylmalonic encephalopathy 1 | XM_030242879.1 | 84.4% | 90.1% | (many diffs) |
| 9 | mouse | 66071 | Ethe1 | ethylmalonic encephalopathy 1 | XM_030242880.1 | 84.4% | 90.1% | (many diffs) |
| 10 | mouse | 66071 | Ethe1 | ethylmalonic encephalopathy 1 | XM_030242881.1 | 84.4% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 828
- ORF length:
- 762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaggctgta ctgagggtcg cccggcggca gctgagccag cgcggcgggt 121 ctggagcccc catcctcctg cggcagatgt tcgagcctgt gagctgcacc ttcacgtacc 181 tgctgggtga cagagagtcc cgggaggccg ttctgatcga cccagtcctg gaaacagcgc 241 ctcgggatgc ccagctgatc aaggagctgg ggctgcggct gctctatgct gtgaataccc 301 actgccacgc ggaccacatt acaggctcgg ggctgctccg ttccctcctc cctggctgcc 361 agtctgtcat ctcccgcctt agtgggGCCC AGGCTGACTT ACACATTGAG GATGGAGACT 421 CCATCCGCTT CGGGCGCTTC GCGTTGGAGA CCAGGGCCAG CCCTGGCCAC ACCCCAGGCT 481 GTGTCACCTT CGTCCTGAAT GACCACAGCA TGGCCTTCAC TGGAGATGCC CTGTTGATCC 541 GTGGGTGTGG GCGGACAGAC TTCCAGCAAG GCTGTGCCAA GACCTTGTAC CACTCGGTCC 601 ATGAAAAGAT CTTCACACTT CCAGGAGACT GTCTGATCTA CCCTGCTCAC GATTACCATG 661 GGTTCACAGT GTCCACCGTG GAGGAGGAGA GGACTCTGAA CCCTCGGCTC ACCCTCAGCT 721 GTGAGGAGTT TGTCAAAATC ATGGGCAACC TGAACTTGCC TAAACCTCAG CAGATAGACT 781 TTGCTGTTCC AGCCAACATG CGCTGTGGGG TGCAGACACC CACTGCCTGC CCAACTTTCT 841 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 901 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 961 GTGGAAAGGA CGAATTGCTC TTCACTATCC AATAACTACG CGTTAAGTCg acaatcaacc 1021 tctggattac aaaatttgtg aaagatt