Construct: ORF TRCN0000471103
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004900.1_s317c1
- Derived from:
- ccsbBroadEn_14380
- DNA Barcode:
- AAAAACCCTATCCACGGGTGAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TAMM41 (132001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471103
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NM_138807.4 | 99.8% | 3% | 9_10insN |
2 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NM_001284401.2 | 93.6% | 4.1% | 9_10insN;874_936del |
3 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | XM_017005725.2 | 93.2% | 2% | (many diffs) |
4 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NM_001321294.2 | 81.1% | 4.6% | (many diffs) |
5 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | XM_017005726.2 | 81% | 2.2% | (many diffs) |
6 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NM_001366031.2 | 74.7% | 2.5% | (many diffs) |
7 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | XM_005264873.4 | 67.6% | 1.4% | (many diffs) |
8 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_158710.1 | 64.2% | (many diffs) | |
9 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | XR_001740021.2 | 62.3% | (many diffs) | |
10 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_135611.2 | 60.1% | (many diffs) | |
11 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | XM_005264875.4 | 59.4% | 8.2% | (many diffs) |
12 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | XM_017005727.1 | 54.4% | 6.7% | (many diffs) |
13 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_135609.2 | 45.4% | (many diffs) | |
14 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NM_001321295.2 | 44.4% | 8% | 0_1ins499;376_438del |
15 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_158713.2 | 41.1% | (many diffs) | |
16 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_158712.1 | 36.8% | (many diffs) | |
17 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_104314.2 | 35.6% | (many diffs) | |
18 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_158711.1 | 31% | (many diffs) | |
19 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | NR_158714.1 | 29.5% | (many diffs) | |
20 | human | 132001 | TAMM41 | TAM41 mitochondrial translo... | XR_001740020.2 | 29% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 234
- ORF length:
- 168
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggaatggc gctgncagac gctgcagagc tcgtgggtga ccttccgcaa gatcctgtct 121 cacttccccg aggagctgag tctggctttc gtctacggct ccggggtgta ccgccaggca 181 gggccgagtt cagaccagaa gaatgctatg ctggactttg tgttcacagt agatgaccct 241 gtcgcatggc attcaaagaa cctgaagaaa aattggagtc actactcttt cctaaaagtt 301 ttagggccca agattatcac gtccatccag aataactatg gcgctggagt ttactacaat 361 tcattgatca tgtgtaatgg taggcttatc aaatatggag ttattagcac taacgttctg 421 attgaagatc tcctcaactg gaataactta tacattgctg gacgactcca aaaaccggtg 481 aaaattatct cagtgaacga ggatgtcact cttagatcag ccctcgatag aaatctgaag 541 agtgctgtga ccgctgcttt cctcatgctc cccgaaagct tttctgaaga agacctcttc 601 atagagattg ccggtctctc ctattcaggt gactttcgga tggtggttgg agaagataaa 661 acaaaagtgt tgaatattgt gaagcccaat atagcccact ttcgagagct ctatggcagc 721 atactacagg aaaatccTCA AGTGGTGTAT AAAAGCCAGC AAGGCTGGCT GGAGATAGAT 781 AAAAGCCCAG AAGGACAGTT CACTCAGCTG ATGACATTGC CCAAAACCTT ACAGCAACAG 841 ATAAATCATA TTATGGACCC TCCTGGAAAA AACAGAGATG TGGAAGAAAC TTTATTCCAA 901 GTGGCTCATG ATCCCGACTG TGGAGATGTG GTGCGACTAG GCCTGAAGAA GTCAGTGATT 961 TATAGTTCAC TAAAACTGCA CAAAATGTGG AAAGGGTGGC TGAGGAAAAC ATCCTGCCCA 1021 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 1081 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 1141 ATATCTTGTG GAAAGGACGA AAAAACCCTA TCCACGGGTG AATCACGCGT TAAGTCgaca 1201 atcaacctct ggattacaaa atttgtgaaa gatt