Construct: ORF TRCN0000471106
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004009.1_s317c1
- Derived from:
- ccsbBroadEn_08779
- DNA Barcode:
- TAGTCCCGGGCCCATATGCATACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF71 (58491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471106
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58491 | ZNF71 | zinc finger protein 71 | NM_001370214.1 | 99.9% | 100% | 936G>T |
| 2 | human | 58491 | ZNF71 | zinc finger protein 71 | NM_021216.4 | 99.9% | 100% | 936G>T |
| 3 | human | 58491 | ZNF71 | zinc finger protein 71 | NM_001370215.1 | 89% | 89% | 1_180del;1116G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1533
- ORF length:
- 1467
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agagttggat ccaaagaatg acatttcgga agacaagctc tccgttgttg 121 gggaggccac ggggggaccc acgaggaatg gtgccagggg tcctggctca gaaggagtgt 181 gggaaccagg cagctggcca gagaggccgc ggggagatgc aggtgcagag tgggagccat 241 tgggaattcc ccaggggaac aaactcttag ggggctcagt acccgcatgt catgaactga 301 aggcatttgc caaccaaggc tgtgtcctgg tcccaccacg gctggacgac cccacagaaa 361 agggggcctg tccacccgta aggcgtggca agaacttctc cagcacttca gacctcagta 421 agccccccat gccctgcgag gagaagaaaa cctacgactg cagcgagtgt ggcaaggcct 481 ttagccgaag ctcgtccctg ataaagcacc aaaggatcca cacgggagaa aagccgtttg 541 agtgtgacac ctgtgggaag cacttcatcg agcgctcgtc cctcaccatc caccagcggg 601 tgcacacggg cgagaagccc tatgcctgcg gggactgcgg caaggccttc agccagcgca 661 tgaacctcac tgtgcaccag cgcacgcaca cgggcgagaa gccgtatgtg tgcgacgtgt 721 gtggcaaggc cttccggaag acttcctctc tcacccagca cgagcggatc cacacggggg 781 agaagcccta cgcgtgcggg gactgcggca aggccttcag ccagaacatg cacctcatcg 841 tgcaccagcg cacgcacacc ggggagaagc cgtacgtgtg ccccgagtgc gggcgagcct 901 tcagccagaa catgcacctg accgagcacc agcgcacgca caccggggag aagccgtacg 961 cctgcaagga gtgcggcaag gccttcaaca agagctcctc tctcaccctg caccagagga 1021 accacaccgg cgagaagccc TACGTGTGCG GCGAGTGCGG CAAGGCCTTC AGCCAGAGCT 1081 CCTACCTCAT CCAGCACCAG CGCTTCCACA TCGGCGTGAA GCCGTTCGAG TGCAGCGAGT 1141 GCGGCAAGGC CTTCAGCAAG AACTCCTCGC TCACGCAGCA CCAGCGCATC CACACCGGCG 1201 AGAAGCCCTA CGAGTGCTAC ATCTGCAAGA AGCACTTCAC GGGGCGCTCG TCCCTCATCG 1261 TGCACCAGAT CGTGCACACC GGGGAGAAGC CCTACGTGTG CGGCGAGTGC GGCAAGGCCT 1321 TCAGCCAGAG CGCCTACCTC ATCGAGCACC AGCGGATCCA CACCGGCGAG AAGCCGTACA 1381 GGTGCGGCCA GTGCGGGAAG TCCTTCATCA AGAACTCCTC CCTCACTGTG CACCAGCGGA 1441 TCCACACGGG CGAGAAGCCC TACCGATGCG GCGAGTGCGG GAAGACCTTC AGCCGCAACA 1501 CGAACCTGAC GCGCCACCTG CGGATTCACA CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1561 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1621 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1681 GTCCCGGGCC CATATGCATA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1741 ttgtgaaaga tt