Construct: ORF TRCN0000471108
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017387.1_s317c1
- Derived from:
- ccsbBroadEn_12528
- DNA Barcode:
- ACGACTATAATCCCTGAATCGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRFN4 (78999)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471108
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 78999 | LRFN4 | leucine rich repeat and fib... | NM_001363524.2 | 51% | 51% | 1_933del |
| 2 | human | 78999 | LRFN4 | leucine rich repeat and fib... | NM_024036.5 | 51% | 51% | 1_933del |
| 3 | mouse | 225875 | Lrfn4 | leucine rich repeat and fib... | NM_153388.4 | 45% | 49.5% | (many diffs) |
| 4 | mouse | 225875 | Lrfn4 | leucine rich repeat and fib... | XM_017318142.1 | 45% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ctgggtcggt cctgacgacc ggttggttgg caactcctcc cgagcccggg 121 ctttccccaa cgggacctta gagattgggg tgaccggcgc tggggacgct gggggctaca 181 cctgcatcgc caccaaccct gctggtgagg ccacagcccg agtagaactg cgggtgctgg 241 ccttgcccca tggtgggaac agcagtgccg aggggggccg ccccgggccc tcggacatcg 301 ccgcctccgc tcgcactgct gccgagggtg aggggacgct ggagtctgag ccagccgtgc 361 aggtgacgga ggtgaccgcc acctcagggc tggtgagctg gggtcccggg cggccagccg 421 acccagtgtg gatgttccaa atccagtaca acagcagcga agatgagacc ctcatctacc 481 ggattgtccc agcctccagc caccacttcc tgctgaagca cctcgtcccc ggcgctgact 541 atgacctctg cctgctggcc ttgtcaccgg ccgctgggcc ctctgaccTC ACGGCCACCA 601 GGCTGCTGGG CTGTGCCCAT TTCTCCACGC TGCCGGCCTC GCCCCTGTGC CACGCCCTGC 661 AGGCCCACGT GCTGGGCGGG ACCCTGACCG TGGCCGTGGG GGGTGTGCTG GTGGCTGCCT 721 TACTGGTCTT CACTGTGGCC TTGCTGGTTC GGGGCCGGGG GGCCGGAAAT GGCCGCCTCC 781 CCCTCAAGCT CAGCCACGTC CAGTCCCAGA CCAATGGAGG CCCCAGCCCC ACACCCAAGG 841 CCCACCCGCC GCGGAGCCCC CCGCCCCGGC CGCAGCGCAG CTGCTCTCTG GACCTGGGAG 901 ATGCCGGGTG CTACGGTTAT GCCAGGCGCC TGGGAGGAGC TTGGGCCCGA CGGAGCCACT 961 CTGTGCATGG GGGGCTGCTC GGGGCAGGGT GCCGGGGGGT AGGAGGCAGC GCCGAGCGGC 1021 TGGAAGAGAG TGTGGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACGACTA TAATCCCTGA 1201 ATCGCACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt