Construct: ORF TRCN0000471124
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014945.1_s317c1
- Derived from:
- ccsbBroadEn_11487
- DNA Barcode:
- CAGGATAGTACGGTATTCATTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCA9 (10350)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471124
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XM_024450531.1 | 13.4% | 13.4% | 1_339del;826_3615del |
| 2 | human | 10350 | ABCA9 | ATP binding cassette subfam... | NM_080283.4 | 9.9% | 9.9% | 1_315del;802_4872del |
| 3 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XM_017024012.1 | 9.9% | 9.9% | 1_315del;802_4878del |
| 4 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XM_017024013.1 | 9.9% | 9.9% | 1_315del;802_4878del |
| 5 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XM_024450530.1 | 9.9% | 9.9% | 1_315del;802_4878del |
| 6 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XM_017024011.1 | 9.9% | 9.9% | 1_339del;826_4896del |
| 7 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XM_024450529.1 | 9.9% | 9.9% | 1_339del;826_4902del |
| 8 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XR_002957943.1 | 8.9% | 1_423del;910_5441del | |
| 9 | human | 10350 | ABCA9 | ATP binding cassette subfam... | XM_017024015.2 | 7.5% | 7.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gtggcctgat gaaaaaagca tggatgaatt ggatttgaac tattcaatag 121 acgcagtgag agtcatcttt actgatacct tctcctacca tttgaagttt tcttggggac 181 atagaaTCCC CATGATGAAA GAGCACAGAG ACCATTCAGC TCACTGTCAA GCAGTGAATG 241 AAAAAATGAA GTGTGAAGGT TCAGAGTTCT GGGAGAAAGG CTTTGTAGCT TTTCAAGCTG 301 CCATTAATGC TGCTATCATA GAAATCGCAA CAAATCATTC AGTGATGGAA CAGCTGATGT 361 CAGTTACTGG TGTACATATG AAGATATTAC CTTTTGTTGC CCAAGGAGGA GTTGCAACTG 421 ATTTTTTCAT TTTCTTTTGC ATTATTTCTT TTTCTACATT TATATACTAT GTATCAGTCA 481 ATGTTACACA AGAAAGACAA TACATTACGT CATTGATGAC AATGATGGGA CTCCGAGAGT 541 CAGCATTCTG GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAG GATAGTACGG TATTCATTTT 721 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t