Construct: ORF TRCN0000471125
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007339.1_s317c1
- Derived from:
- ccsbBroadEn_00012
- DNA Barcode:
- CCTGACGCATTCCAATTTCCGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACP5 (54)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471125
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54 | ACP5 | acid phosphatase 5, tartrat... | NM_001111034.3 | 100% | 100% | |
| 2 | human | 54 | ACP5 | acid phosphatase 5, tartrat... | NM_001111035.3 | 100% | 100% | |
| 3 | human | 54 | ACP5 | acid phosphatase 5, tartrat... | NM_001111036.3 | 100% | 100% | |
| 4 | human | 54 | ACP5 | acid phosphatase 5, tartrat... | NM_001322023.2 | 100% | 100% | |
| 5 | human | 54 | ACP5 | acid phosphatase 5, tartrat... | NM_001611.5 | 100% | 100% | |
| 6 | human | 54 | ACP5 | acid phosphatase 5, tartrat... | XM_005259938.1 | 100% | 100% | |
| 7 | human | 54 | ACP5 | acid phosphatase 5, tartrat... | XM_011528069.2 | 100% | 100% | |
| 8 | mouse | 11433 | Acp5 | acid phosphatase 5, tartrat... | NM_001102404.1 | 81.3% | 85% | (many diffs) |
| 9 | mouse | 11433 | Acp5 | acid phosphatase 5, tartrat... | NM_001102405.1 | 81.3% | 85% | (many diffs) |
| 10 | mouse | 11433 | Acp5 | acid phosphatase 5, tartrat... | NM_007388.3 | 81.3% | 85% | (many diffs) |
| 11 | mouse | 11433 | Acp5 | acid phosphatase 5, tartrat... | XM_006509944.3 | 81.3% | 85% | (many diffs) |
| 12 | mouse | 11433 | Acp5 | acid phosphatase 5, tartrat... | XM_006509945.2 | 81.3% | 85% | (many diffs) |
| 13 | mouse | 11433 | Acp5 | acid phosphatase 5, tartrat... | XM_006509946.1 | 81.3% | 85% | (many diffs) |
| 14 | mouse | 11433 | Acp5 | acid phosphatase 5, tartrat... | XM_011242384.2 | 81.3% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga catgtggacg gcgctgctca tcctgcaagc cttgttgcta ccctccctgg 121 ctgatggtgc cacccctgcc ctgcgctttg tagccgtggg tgactgggga ggggtcccca 181 atgccccatt ccacacggcc cgggaaatgg ccaatgccaa ggagatcgct cggactgtgc 241 agatcctggg tgcagacttc atcctgtctc taggggacaa tttttacttc actggtgtgc 301 aagacatcaa tgacaagagg ttccaggaga cctttgagga cgtattctct gaccgctccc 361 ttcgcaaagt gccctggtac gtgctagccg gaaaccatga ccaccttggc aatgtctctg 421 cccagattgc atactctaag atctccaagc gctggaactt ccccagccct ttctaccgcc 481 tgcacttcaa gatcccacag accaatgtgt ctgtggccat ttttatgctg gacacagtga 541 cactatgtgg caactcagat gacttcctca gccagcagcc tgagaggccc cgagacgtga 601 agctggcccg cacacagctg tcctggctca agaaacagct GGCGGCGGCC AGGGAGGACT 661 ACGTGCTGGT GGCTGGCCAC TACCCCGTGT GGTCCATAGC CGAGCACGGG CCTACCCACT 721 GCCTGGTCAA GCAGCTACGG CCACTGCTGG CCACATACGG GGTCACTGCC TACCTGTGCG 781 GCCACGATCA CAATCTGCAG TACCTGCAAG ATGAGAATGG CGTGGGCTAC GTGCTGAGTG 841 GGGCTGGGAA TTTCATGGAC CCCTCAAAGC GGCACCAGCG CAAGGTCCCC AACGGCTATC 901 TGCGCTTCCA CTATGGGACT GAAGACTCAC TGGGTGGCTT TGCCTATGTG GAGATCAGCT 961 CCAAAGAGAT GACTGTCACT TACATCGAGG CCTCGGGCAA GTCCCTCTTT AAGACCAGGC 1021 TGCCGAGGCG AGCCAGGCCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCTG ACGCATTCCA 1201 ATTTCCGTCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt