Construct: ORF TRCN0000471127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003447.1_s317c1
- Derived from:
- ccsbBroadEn_12673
- DNA Barcode:
- GACCGAGCTCCGCCATCCGTGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASRGL1 (80150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | XM_005274305.4 | 100% | 100% | |
| 2 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | XM_005274306.2 | 100% | 100% | |
| 3 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | NM_001083926.2 | 58.4% | 58.4% | 1_384del |
| 4 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | NM_025080.4 | 58.4% | 58.4% | 1_384del |
| 5 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | XR_001747982.2 | 32.7% | 1_540del;765_786del;1103_1647del | |
| 6 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | XM_011545265.3 | 27.4% | 25% | (many diffs) |
| 7 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | XR_002957198.1 | 21.4% | 1_540del;876_1177del;1383_2516del | |
| 8 | human | 80150 | ASRGL1 | asparaginase and isoasparty... | XR_002957199.1 | 9.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 606
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggttccagag attcctggag aaaaactggt gacagagaga aacaaaaagc 121 gcctggaaaa agagaagcat gaaaaaggtg ctcagaaaac agattgtcaa aaaaacttgg 181 gaaccgtggg tgctgttgcc ttggactgca aagggaatgt agccTACGCA ACCTCCACAG 241 GCGGTATCGT TAATAAAATG GTCGGCCGCG TTGGGGACTC ACCGTGTCTA GGAGCTGGAG 301 GTTATGCCGA CAATGACATC GGAGCCGTCT CAACCACAGG GCATGGGGAA AGCATCCTGA 361 AGGTGAACCT GGCTAGACTC ACCCTGTTCC ACATAGAACA AGGAAAGACG GTAGAAGAGG 421 CTGCGGACCT ATCGTTGGGT TATATGAAGT CAAGGGTTAA AGGTTTAGGT GGCCTCATCG 481 TGGTTAGCAA AACAGGAGAC TGGGTGGCAA AGTGGACCTC CACCTCCATG CCCTGGGCAG 541 CCGCCAAGGA CGGCAAGCTG CACTTCGGAA TTGATCCTGA CGATACTACT ATCACCGACC 601 TTCCCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 721 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGACCGAGCT CCGCCATCCG TGTACACGCG 781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt