Construct: ORF TRCN0000471136
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001170.1_s317c1
- Derived from:
- ccsbBroadEn_12877
- DNA Barcode:
- ATAAATCCTGATCGTGATAGCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFATC2IP (84901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471136
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84901 | NFATC2IP | nuclear factor of activated... | NM_032815.3 | 32.9% | 32.9% | 1_843del |
| 2 | human | 84901 | NFATC2IP | nuclear factor of activated... | XR_950864.3 | 15.9% | (many diffs) | |
| 3 | mouse | 18020 | Nfatc2ip | nuclear factor of activated... | NM_010900.3 | 29.1% | 29.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGTC GGAGCCCCTG CAGAGTGTGG TGGACCACAT GGCCACCCAC CTTGGGGTGT 121 CCCCAAGCAG GATCCTTTTG CTTTTTGGAG AGACAGAGCT ATCACCTACT GCCACTCCCA 181 GGACCCTAAA GCTCGGAGTG GCTGACATCA TTGACTGTGT GGTACTAACA AGTTCTCCAG 241 AGGCCACAGA GACGTCCCAA CAGCTCCAGC TCCGGGTGCA GGGAAAGGAG AAACACCAGA 301 CACTGGAAGT CTCACTGTCT CGAGATTCCC CTCTAAAGAC CCTCATGTCC CACTATGAGG 361 AGGCCATGGG ACTGTCGGGA CGGAAGCTCT CCTTCTTCTT TGATGGGACA AAGCTTTCAG 421 GCAGGGAGCT GCCAGCTGAC CTGGGCATGG AATCTGGGGA CCTCATTGAG GTCTGGGGCT 481 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGAATAAA TCCTGATCGT GATAGCTTAA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt