Construct: ORF TRCN0000471148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014390.1_s317c1
- Derived from:
- ccsbBroadEn_12589
- DNA Barcode:
- CCCAAAGTTCAACGAAATGAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NLRX1 (79671)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79671 | NLRX1 | NLR family member X1 | XM_005271670.1 | 84.5% | 81.1% | (many diffs) |
| 2 | human | 79671 | NLRX1 | NLR family member X1 | XM_005271671.1 | 84.5% | 81.1% | (many diffs) |
| 3 | human | 79671 | NLRX1 | NLR family member X1 | XM_005271672.1 | 84.5% | 81.1% | (many diffs) |
| 4 | human | 79671 | NLRX1 | NLR family member X1 | XM_006718904.1 | 75% | 75% | 1_687del;2378C>A |
| 5 | human | 79671 | NLRX1 | NLR family member X1 | NM_001282143.2 | 69.1% | 66.3% | (many diffs) |
| 6 | human | 79671 | NLRX1 | NLR family member X1 | NM_001282144.2 | 69.1% | 66.3% | (many diffs) |
| 7 | human | 79671 | NLRX1 | NLR family member X1 | NM_001282358.1 | 69.1% | 66.3% | (many diffs) |
| 8 | human | 79671 | NLRX1 | NLR family member X1 | NM_024618.4 | 69.1% | 66.3% | (many diffs) |
| 9 | human | 79671 | NLRX1 | NLR family member X1 | XM_005271669.1 | 69.1% | 66.3% | (many diffs) |
| 10 | human | 79671 | NLRX1 | NLR family member X1 | XM_011542980.1 | 69.1% | 66.3% | (many diffs) |
| 11 | human | 79671 | NLRX1 | NLR family member X1 | XM_017018294.2 | 35.9% | 36.4% | 1_687del;1670_1730del;1764_1765ins1060 |
| 12 | mouse | 270151 | Nlrx1 | NLR family member X1 | NM_001163742.1 | 60.1% | 61.5% | (many diffs) |
| 13 | mouse | 270151 | Nlrx1 | NLR family member X1 | NM_001163743.1 | 60.1% | 61.5% | (many diffs) |
| 14 | mouse | 270151 | Nlrx1 | NLR family member X1 | NM_178420.3 | 60.1% | 61.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2142
- ORF length:
- 2076
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgctgctggg tcccacctcc tctttgtgct ccatggctta gagcatctca 121 acctcgactt ccggctggca ggcacgggac tttgtagtga cccggaggaa ccgcaggaac 181 cagctgctat catcgtcaac ctgctgcgca aatacatgct gcctcaggcc agcattctgg 241 tgaccactcg gccctctgcc attggccgta tccccagcaa gtacgtgggc cgctatggtg 301 agatctgcgg tttctctgat accaacctgc agaagctcta cttccagctc cgcctcaacc 361 agccgtactg cgggtatgcc gttggcggtt caggtgtctc tgccacacca gctcagcgtg 421 accacctggt gcagatgctc tcccggaacc tggaggggca ccaccagata gccgctgcct 481 gcttcctgcc gtcctattgc tggctcgttt gtgccacctt gcacttcctg catgccccca 541 cgcctgctgg gcagaccctt acaagcatct ataccagctt cctgcgcctc aacttcagcg 601 gggaaaccct ggacagcact gacccctcca atttgtccct gatggcctat gcagcccgaa 661 ccatgggcaa gttggcctat gagggggtgt cctcccgcaa gacctacttc tctgaagagg 721 atgtctgtgg ctgcctggag gctggcatca ggacggagga ggagtttcag ctgctgcaca 781 tcttccgtcg ggatgccctg aggtttttcc tggccccatg tgtggagcca gggcgtgcag 841 gcaccttcgt gttcaccgtg cccgccatgc aggaatacct ggctgccctc tacattgtgc 901 tgggtttgcg caagacgacc ctgcaaaagg tgggcaagga agtggctgag ctcgtgggcc 961 gtgttgggga ggacgtcagc ctggtactgg gcatcatggc caagctgctg cctctgcggg 1021 ctctgcctct gctcttcaac ctgatcaagg tggttccacg agtgtttggg cgcatggtgg 1081 gtaaaagccg ggaggcggtg gctcaggcca tggtgctgga gatgtttcga gaggaggact 1141 actacaacga tgatgttctg gaccagatgg gcgccagtat cctgggcgtg gagggccccc 1201 ggcgccaccc agatgagccc cctgaggatg aagtcttcga gctcttcccc atgttcatgg 1261 gggggcttct ctctgcccac aaccgagctg tgctagctca gcttggctgc cccatcaaga 1321 acctggatgc cctggagaat gcccaggcca tcaagaagaa gctgggcaag ctgggccggc 1381 aggtgctgcc cccatcagag ctccttgacc acctcttctt ccactatgag ttccagaacc 1441 agcgcttctc cgctgaggtg ctcagctccc tgcgtcagct caacctggca ggtgtgcgca 1501 tgacaccagt caagtgcaca gtggtggcag ctgtgctggg cagcggaagg catgccctgg 1561 atgaggtgaa cttggcctcc tgccagctag atcctgctgg gctgcgcaca ctcctgcctg 1621 tcttcctgcg tgcccggaag ctgggcttgc aactcaacag cctgggccct gaggcctgca 1681 aggacctccg agacctgttg ctgcatgacc agtgccaaat taccacactg cggctgtcca 1741 acaacccgct gacggaggca ggtgttgccg tgctaatgga ggggctggca ggaaacacct 1801 CAGTGACGCA CCTGTCCCTG CTGCACACGG GCCTTGGGGA CGAAGGCCTG GAGCTGCTGG 1861 CTGCCCAGCT GGACCGCAAC CGGCAGCTGC AGGAGCTGAA CGTGGCGTAC AACGGTGCTG 1921 GTGACACAGC GGCCCTGGCC CTGGCCAGAG CTGCCCGGGA GCACCCTTCC CTGGAACTGC 1981 TACAGGGTGT CGCCATCCAG ATGTGTTGGA AGCTTCCCCT CCTGCCTTAT GCTCACCTGT 2041 GGACACCGAG GATGCCCTCA CATTGGTGCT TTCTCCTCAT CCTCATGCCC CCTTTGCCAC 2101 AATGGTATGA TGGCTTGGTA GCCCCTCGAG GCAGATGCAC CTGCCCAACT TTCTTGTACA 2161 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2221 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2281 AGGACGACCC AAAGTTCAAC GAAATGAAAT AACGCGTTAA GTCgacaatc aacctctgga 2341 ttacaaaatt tgtgaaagat t