Construct: ORF TRCN0000471176
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010950.1_s317c1
- Derived from:
- ccsbBroadEn_02366
- DNA Barcode:
- CACTCATGCGAATTCTGACGGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF7 (10238)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471176
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10238 | DCAF7 | DDB1 and CUL4 associated fa... | NM_005828.5 | 100% | 100% | |
| 2 | human | 10238 | DCAF7 | DDB1 and CUL4 associated fa... | NR_073585.2 | 75.4% | 1_201del;1228_1359del | |
| 3 | mouse | 71833 | Dcaf7 | DDB1 and CUL4 associated fa... | NM_027946.3 | 92% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1092
- ORF length:
- 1026
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cctgcacggc aaacggaagg agatctacaa gtatgaagcg ccctggacag 121 tctacgcgat gaactggagt gtgcggcccg ataagcgctt tcgcttggcg ctgggcagct 181 tcgtggagga gtacaacaac aaggttcagc ttgttggttt agatgaggag agttcagagt 241 ttatttgcag aaacaccttt gaccacccat accccaccac aaagctcatg tggatccctg 301 acacaaaagg cgtctatcca gacctactgg caacaagcgg tgactatctc cgtgtgtgga 361 gggttggtga aacagagacc aggctggagt gtttgctaaa caataataag aactctgatt 421 tctgtgctcc cctgacctcc tttgactgga atgaggtgga tccttatctt ttaggtacct 481 caagcattga tacgacatgc accatctggg ggctggagac agggcaggtg ttagggcgag 541 tgaatctcgt gtctggccac gtgaagaccc agctgatcgc ccatgacaaa gaggtctatg 601 atattgcatt tagccgggcc gggggtggca gggacatgtt tgcctctgtg ggtgctgatg 661 gctcggtgcg gatgtttgac cTCCGCCATC TAGAACACAG CACCATCATT TACGAAGACC 721 CACAGCATCA CCCACTGCTT CGCCTCTGCT GGAACAAGCA GGACCCTAAC TACCTGGCCA 781 CCATGGCCAT GGATGGAATG GAGGTGGTGA TTCTAGATGT CCGGGTTCCC TGCACACCTG 841 TCGCCAGGTT AAACAACCAT CGAGCATGTG TCAATGGCAT TGCTTGGGCC CCACATTCAT 901 CCTGCCACAT CTGCACTGCA GCGGATGACC ACCAGGCTCT CATCTGGGAC ATCCAGCAAA 961 TGCCCCGAGC CATTGAGGAC CCTATCCTGG CCTACACAGC TGAAGGAGAG ATCAACAATG 1021 TGCAGTGGGC ATCAACTCAG CCCGACTGGA TCGCCATCTG CTACAACAAC TGCCTGGAGA 1081 TACTCAGAGT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1141 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1201 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAC TCATGCGAAT TCTGACGGGG 1261 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t