Construct: ORF TRCN0000471176

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010950.1_s317c1
Derived from:
ccsbBroadEn_02366
DNA Barcode:
CACTCATGCGAATTCTGACGGGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCAF7 (10238)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471176
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10238 DCAF7 DDB1 and CUL4 associated fa... NM_005828.5 100% 100%
2 human 10238 DCAF7 DDB1 and CUL4 associated fa... NR_073585.2 75.4% 1_201del;1228_1359del
3 mouse 71833 Dcaf7 DDB1 and CUL4 associated fa... NM_027946.3 92% 100% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1092
ORF length:
1026
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc cctgcacggc aaacggaagg agatctacaa gtatgaagcg ccctggacag
121tctacgcgat gaactggagt gtgcggcccg ataagcgctt tcgcttggcg ctgggcagct
181tcgtggagga gtacaacaac aaggttcagc ttgttggttt agatgaggag agttcagagt
241ttatttgcag aaacaccttt gaccacccat accccaccac aaagctcatg tggatccctg
301acacaaaagg cgtctatcca gacctactgg caacaagcgg tgactatctc cgtgtgtgga
361gggttggtga aacagagacc aggctggagt gtttgctaaa caataataag aactctgatt
421tctgtgctcc cctgacctcc tttgactgga atgaggtgga tccttatctt ttaggtacct
481caagcattga tacgacatgc accatctggg ggctggagac agggcaggtg ttagggcgag
541tgaatctcgt gtctggccac gtgaagaccc agctgatcgc ccatgacaaa gaggtctatg
601atattgcatt tagccgggcc gggggtggca gggacatgtt tgcctctgtg ggtgctgatg
661gctcggtgcg gatgtttgac cTCCGCCATC TAGAACACAG CACCATCATT TACGAAGACC
721CACAGCATCA CCCACTGCTT CGCCTCTGCT GGAACAAGCA GGACCCTAAC TACCTGGCCA
781CCATGGCCAT GGATGGAATG GAGGTGGTGA TTCTAGATGT CCGGGTTCCC TGCACACCTG
841TCGCCAGGTT AAACAACCAT CGAGCATGTG TCAATGGCAT TGCTTGGGCC CCACATTCAT
901CCTGCCACAT CTGCACTGCA GCGGATGACC ACCAGGCTCT CATCTGGGAC ATCCAGCAAA
961TGCCCCGAGC CATTGAGGAC CCTATCCTGG CCTACACAGC TGAAGGAGAG ATCAACAATG
1021TGCAGTGGGC ATCAACTCAG CCCGACTGGA TCGCCATCTG CTACAACAAC TGCCTGGAGA
1081TACTCAGAGT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1141ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1201CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAC TCATGCGAAT TCTGACGGGG
1261GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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