Construct: ORF TRCN0000471181
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007830.1_s317c1
- Derived from:
- ccsbBroadEn_02001
- DNA Barcode:
- ATACTGTTTACAGTACCGCGCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNX3 (8724)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471181
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8724 | SNX3 | sorting nexin 3 | NM_003795.6 | 100% | 100% | |
| 2 | human | 8724 | SNX3 | sorting nexin 3 | NM_001300929.2 | 86.4% | 86.4% | 96_97ins66 |
| 3 | human | 8724 | SNX3 | sorting nexin 3 | NM_152827.4 | 80.2% | 80.2% | 158_159ins96 |
| 4 | human | 8724 | SNX3 | sorting nexin 3 | NM_001300928.2 | 64.8% | 52.9% | 258_259ins125;315_316ins46 |
| 5 | mouse | 54198 | Snx3 | sorting nexin 3 | NM_017472.4 | 93.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagaccgtg gctgacaccc ggcggctgat caccaagccg cagaacctga 121 atgacgccta cggaCCCCCC AGCAACTTCC TCGAGATCGA TGTGAGCAAC CCGCAAACGG 181 TGGGGGTCGG CCGGGGCCGC TTCACCACTT ACGAAATCAG GGTCAAGACA AATCTTCCTA 241 TTTTCAAGCT GAAAGAATCT ACTGTTAGAA GAAGATACAG TGACTTTGAA TGGCTGCGAA 301 GTGAATTAGA AAGAGAGAGC AAGGTCGTAG TTCCCCCGCT CCCTGGGAAA GCGTTTTTGC 361 GTCAGCTTCC TTTTAGAGGA GATGATGGAA TATTTGATGA CAATTTTATT GAGGAAAGAA 421 AACAAGGGCT GGAGCAGTTT ATAAACAAGG TCGCTGGTCA TCCTCTGGCA CAGAACGAAC 481 GTTGTCTTCA CATGTTTTTA CAAGATGAAA TAATAGATAA AAGCTATACT CCATCTAAAA 541 TAAGACATGC CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATA CTGTTTACAG TACCGCGCAG 721 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t