Construct: ORF TRCN0000471187
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009671.1_s317c1
- Derived from:
- ccsbBroadEn_08058
- DNA Barcode:
- ATCACCTTAGTCCGGTATGTAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DKK2 (27123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471187
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27123 | DKK2 | dickkopf WNT signaling path... | NM_014421.3 | 99.8% | 99.6% | 437G>A |
| 2 | mouse | 56811 | Dkk2 | dickkopf WNT signaling path... | NM_020265.4 | 89.4% | 94.9% | (many diffs) |
| 3 | mouse | 56811 | Dkk2 | dickkopf WNT signaling path... | XM_006501818.4 | 85.1% | 90.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 846
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgcgttg atgcggagca aggattcgtc ctgctgcctg ctcctactgg 121 ccgcggtgct gatggtggag agctcacaga tcggcagttc gcgggccaaa ctcaactcca 181 tcaagtcctc tctgggcggg gagacgcctg gtcaggccgc caatcgatct gcgggcatgt 241 accaaggact ggcattcggc ggcagtaaga agggcaaaaa cctggggcag gcctaccctt 301 gtagcagtga taaggagtgt gaagttggga ggtattgcca cagtccccac caaggatcat 361 cggcctgcat ggtgtgtcgg agaaaaaaga agcgctgcca ccgagatggc atgtgctgcc 421 ccagtacccg ctgcaataat ggcatctgta tcccagttac tgaaagcaTC TTAACCCCTC 481 ACATCCCGGC TCTGGATGGT ACTCAGCACA GAGATCGAAA CCACGGTCAT TACTCAAACC 541 ATGACTTGGG ATGGCAGAAT CTAGGAAGAC CACACACTAA GATGTCACAT ATAAAAGGGC 601 ATGAAGGAGA CCCCTGCCTA CGATCATCAG ACTGCATTGA AGGGTTTTGC TGTGCTCGTC 661 ATTTCTGGAC CAAAATCTGC AAACCAGTGC TCCATCAGGG GGAAGTCTGT ACCAAACAAC 721 GCAAGAAGGG TTCTCATGGG CTGGAAATTT TCCAGCGTTG CGACTGTGCG AAGGGCCTGT 781 CTTGCAAAGT ATGGAAAGAT GCCACCTACT CCTCCAAAGC CAGACTCCAT GTGTGTCAGA 841 AAATTTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 901 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 961 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCACCTTA GTCCGGTATG TAAACACGCG 1021 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt