Construct: ORF TRCN0000471196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003524.1_s317c1
- Derived from:
- ccsbBroadEn_03407
- DNA Barcode:
- TGGACTCTATGCCGTACTACTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TREM1 (54210)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471196
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54210 | TREM1 | triggering receptor express... | NM_018643.5 | 100% | 100% | |
| 2 | human | 54210 | TREM1 | triggering receptor express... | XM_017010956.2 | 100% | 100% | |
| 3 | human | 54210 | TREM1 | triggering receptor express... | NM_001242589.3 | 90.9% | 85.8% | (many diffs) |
| 4 | human | 54210 | TREM1 | triggering receptor express... | XM_011514696.2 | 85.7% | 77.3% | (many diffs) |
| 5 | human | 54210 | TREM1 | triggering receptor express... | NM_001242590.3 | 64.1% | 57.6% | 405_406ins193;450_451ins59 |
| 6 | human | 54210 | TREM1 | triggering receptor express... | XM_017010957.1 | 64.1% | 57.6% | 405_406ins193;450_451ins59 |
| 7 | human | 54210 | TREM1 | triggering receptor express... | XM_006715117.3 | 59.1% | 58.1% | (many diffs) |
| 8 | human | 54210 | TREM1 | triggering receptor express... | XM_011514697.1 | 58.1% | 58.1% | 408_409ins294 |
| 9 | human | 54210 | TREM1 | triggering receptor express... | NR_136332.1 | 27.5% | 1_90del;793_2547del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gaagaccagg ctctgggggc tgctgtggat gctctttgtc tcagaactcc 121 gagctgcaac taaattaact gaggaaaagt atgaactgaa agaggggcag accctggatg 181 tgaaatgtga ctacacgcta gagaagtttg ccagcagcca gaaagcttgg cagataataa 241 gggacggaga gatgcccaag accctggcat gcacagagag gccttcaaag aattcccatc 301 cagtccaagt ggggaggatc atactagaag actaccatga tcatggttta ctgcgcgtcc 361 gaatggtcaa ccttcaagtg gaagattctg gactgtatca gtgtgtgatc taccagccTC 421 CCAAGGAGCC TCACATGCTG TTCGATCGCA TCCGCTTGGT GGTGACCAAG GGTTTTTCAG 481 GGACCCCTGG CTCCAATGAG AATTCTACCC AGAATGTGTA TAAGATTCCT CCTACCACCA 541 CTAAGGCCTT GTGCCCACTC TATACCAGCC CCAGAACTGT GACCCAAGCT CCACCCAAGT 601 CAACTGCCGA TGTCTCCACT CCTGACTCTG AAATCAACCT TACAAATGTG ACAGATATCA 661 TCAGGGTTCC GGTGTTCAAC ATTGTCATTC TCCTGGCTGG TGGATTCCTG AGTAAGAGCC 721 TGGTCTTCTC TGTCCTGTTT GCTGTCACGC TGAGGTCATT TGTACCCTAC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGATGGACTC TATGCCGTAC TACTTGCACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt